PCR技术检测编码绿脓杆菌外毒素A基因

Detection of the Gene Encoding Exotoxin A of Pseudomonas Aeruginosa by PCR Technique

根据编码绿脓杆菌外毒素A基因设计引物,应用PCR技术检测绿脓杆菌外毒素A基因。从绿脓杆菌中扩增出402bp外毒素A基因。测序结果表明,与已发表序列同源性为100%。PCR敏感度试验表明,可检测到含量为3cfu/mL的绿脓杆菌。从绿脓杆菌、金黄色葡萄球菌、大肠杆菌、乳酸菌菌液中特异性地检出编码绿脓杆菌外毒素A基因。该技术具有简易、敏感、快速和特异性强等特点,可用于食品和细菌感染的绿脓杆菌菌株的鉴定。

Primers were designed according to the encoding gene of Pseudomonas aeruginosa exotoxin A, and Pseudomonas aeruginosa exotoxin A gene was detected by PCR technique. The 402 bp exotoxin A gene was amplified from Pseudomonas aerug/nosa. The identity was 100% between cloned gene and published gene after sequencing. The sensitivity experiments showed that the 3 cfu/mL concentration of Pseudomonas aeruglnosa would be detected. The encoding gene of Pseudomonas aeruginosa exotoxin A could be specifically detected from Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Lactobacillus by the PCR detection technology. It is a specific, sensitive, quick and efficient way of detecting Pseudomonas aeruginosa exotoxin A gene by PCR technology. It provides a useful tool for the Pseudomonas aeruginosa diagnosis in microorganisms infection beth in food and infection.