不同预处理方式对萝卜小孢子活力的影响

Effect of Different Pretreatment on the Vigor of Microspores in Radish(Raphanus sativus L)

研究温度(低温和高温)及培养基(无碳源、甘露醇及秋水仙素)预处理对4个萝卜基因型小孢子活力的影响,得出的结果是:4℃低温条件下处理3 d的小孢子可保持较高的存活率,36℃高温处理后的小孢子存活率明显低于对照;加入蔗糖的培养基对提高小孢子存活率作用明显;甘露醇及秋水仙素处理也能提高萝卜小孢子存活率,其合适的浓度在不同萝卜基因型中存在差异。

Effects of low temperature, high temperature, sugar-free, colchicines and mannitol pretreatment were studied on the survival rate of microspores in 4 radish cuhivars. The result show that microspores had high survival rate under 4℃ after 3 days treatment, while 36℃ treatment reduced the survival rate. The media with sugar, mannitol and colchicines enhanced the vigor of microspores. And the concentrations of mannitol and colchicines had different effects on 4 radish cultivars.