运用PCR技术对人骨组织和牙齿进行性别鉴定的应用研究

The Application of DNA Amplification in vitro to Sex Determination of Human Bone and Tooth

为了解决刑事案件中利用人骨组织和牙齿进行性别鉴定的问题，我们通过骨骼和牙齿中ＤＮＡ的提取床用Ｙ染色体特异ＤＮＭ列引物扩增Ｙ染色体的ＤＹＺＩ和ＳＲＹ基因序列．结果：１．骨组织中ＤＮＡ的提取可在１小时内完成；牙齿中ＤＮＡ的提取可在４小时内完成；２．牙齿中的ＤＮＡ保存的完好性大于骨组织；４．煮沸过的骨组织中的ＤＮＡ降解严重，无法用于实验．

To determine the sex of Human bone and tooth,the DYZI and the SRY,the Y specific sequences were amplified by Polymerase Chain Reaction(PCR) with two sets of Primers and DNA isolated from bones and tooth.Results showed that;1 DNA isolation can be finished within 1 hour and 4 hours for bone and tooth respeCtively; 2.The PCR procedures can be achieved within 1.5 hours,3.The quality of DNA from tooth is better than it from bone,4.The sex determination of DNA from boilled bone failed of success because of the DNA severe dead-adation.