菌斑及其产物对豚鼠牙周组织的影响

The effects of LPS,LTA and plaque on the periodontal tissue of the Guinea pig

４０只豚鼠分成４组，在下切牙区龈沟分别灌注Ｇ－菌内毒素（ＬＰＳ）、Ｇ＋菌脂磷壁酸（ＬＴＡ）、龈下菌斑（ＰＬ）及生理盐水（ＮＳ），於１、２、３、６、８周分批处死观察。结果：ＮＳ对照组除１、２周牙龈出现少量炎症细胞外，未见其他异常；ＬＰＳ组１周很快出现牙龈炎症和明显骨吸收，４、５周炎症渐消退并有新骨形成；ＬＴＡ组菌斑和牙石沉积明显，而炎症与骨吸收较弱，３周即渐恢复；ＰＬ组既见菌斑牙石形成，又有炎症反应及骨吸收，持续时间较长，６、８周仍见轻度炎症。它们作用于牙周组织的不同特点，为进一步阐明牙周病的发病机理提供实验依据。

Abstract Guinea pigs were divided into 4 groups.The gingival sulcus of 2 mandibular incisors were irrigated with LPS (Lipopolysaccharide),LTA (Lipoteichoic acid),PL(Subgingival plaque) and NS(Normal saline solution)respectively.2 animals were sacrificed in group of 5 successively at 1,2,3,6,8 week after irrigation. Microscopic examination demonstrated: Only a few penetration of inflammatory cells lay in the gingival tissue in NS control group. Significant infiltrate associated with evident crestal resorption were noted at 1st week and active remodeling took place at 4th,5th week in LPS group. Enhancing accumulations of plaque calculus with weak inflammation could be seen in LTA group. Not only accumulations of plaque and calculus but also penetration of inflammation with resorption of bone were seen in PL group.Their effects on the periodontal tissue of Guinea pig showed evident different. These findings served further expression the possible mechanisms of periododntal diseases.