高脂血症大鼠血液流变学及肝脏微循环的改变

The Changes of Hemorrheology and Hepatic Microcirculation in Hyperlipoidemia Rats

目的探讨高脂血症大鼠血液流变学及肝脏微循环的改变。方法W istar大鼠随机分为(1)正常组:喂普通饲料;(2)高脂血症组:喂高脂饲料4周,每组6只。于造模4周后测定血清血脂水平、体质量及肝质量与体质量之比、血液流变学和肝脏活体微循环的改变。结果喂高脂饲料4周后肝脏体积增大,体质量及肝质量明显高于正常对照组(P<0.01)。肝组织标本显示肝细胞肿胀、脂肪性变,肝细胞的胞浆内充满大小不等的脂滴。血清胆固醇、低密度脂蛋白较正常对照组明显增高(P<0.01),高密度脂蛋白减少,甘油三脂变化不大;血压及血浆黏度较正常组增高明显(P<0.05);肝脏活体微循环观察显示肝脏微血管数明显减少,肝细胞索增大,泡沫状突起压迫周围血管,血流速度减慢,血管内有红细胞聚集和白细胞黏附。结论短期内高脂食物的摄入可造成脂质代谢紊乱,继而发生血液流变学及肝脏微循环障碍。

Objective To investigate the changes of hemorrheology and hepatic mierocirculation in hyperlipoidemia rats. Methods 12 wistar rats were randomly divided into two groups. （1） Control group were fed with normal diet. （2） hyperlipoidemia group were fed with high cholesterol diet for four weeks. Body weight, weight of liver, serum lipid and hemorrheologic indexes were measured and hepatic mierocirculation was observed. Results Compared with control group, the serum levels of total cholesterol（TC） and low density lipoprotein（LDL-e） increased obviously in group （2）（P 〈 0.01）. However, the serum levels of high density lipoprotein （HDL-c） and glycertriacetate（TG） were not obvoiously changed（P 〉 0. 05）. Body weight, weight of liver, blood pressure, and plasma viscosity were increased significantly than those in control group respectivelly（ P 〈 0.01）. Hepatic microcirculation obviously changed in group （2）. It showed that the microvessls and the blood flow velocity decreased. There was blood cell aggregation and leucocyte adhesion in microvessls. Conclusion High cholesterol diet for four weeks can cause heperlipoidemia, which led to changes of hemon＇heology and hepatic microcirculatioa disorder.