摘要
目的探讨雌激素对血清饥饿诱导的体外培养大鼠成骨细胞凋亡基因bc l-2、bax、fas表达的影响,为明确雌激素抑制由血清饥饿诱导的大鼠成骨细胞凋亡的机理提供依据。方法新生SD大鼠颅骨第2、3代成骨细胞随机分为对照组、血清饥饿组、血清饥饿+雌激素组3组,每组细胞分别培养1 d、2 d、3 d、5 d、7 d、14 d后做免疫组化染色,计数各组Bc l-2、Bax、Fas阳性细胞率。结果对照组成骨细胞有少量Bc l-2、Bax、Fas表达;与对照组比较,血清饥饿组细胞Bax和Fas表达升高(P<0.05),高峰期为14 d,Bc l-2的表达无明显变化(P>0.05);与血清饥饿组比较,血清饥饿+雌激素组细胞Bax和Fas表达降低(P<0.05),高峰期仍为14d,Bc l-2的表达升高(P<0.05)。结论血清饥饿使成骨细胞Bax和Fas表达增强,而对Bc l-2的表达无明显影响;雌激素能抑制血清饥饿增强成骨细胞Bax和Fas表达的作用,并使Bc l-2的表达增加,从而抑制血清饥饿诱导的大鼠成骨细胞凋亡。
Objective To explore the mechanism of estrogen inhibiting osteoblast apoptosis induced with serum hungry. Methods Osteoblasts of the second or third generation from newly born SD rats calvaria were divided randomly into three groups： control group, serum hungry group; serum hungry with estrogen group. Cells of each group were incubated for 1,2, 3,5, 7 or 14 d, and then were stained immunohistochemically. The rates of positive cells of each group were analyzed. Results There was a little positive expression of Bax, Bcl-2 and Fas in control group. The expression of Bax and Fas were significantly increased（P 〈 0. 05）in serum hungry group, peak time was 14 d, but the expression! of Bcl-2 were not affected. Compared with that of serum hungry group, the expression of Bax and Fas significantly decreased （P 〈0. 05.） in serum hungry and estrogen group, peak time was still 14 d, while that of Bcl-2 increased（ P 〈 0.05）. Conclusion Serum hungry can increase the expression of Bax and Fas in osteoblast, that can be inhibited by estrogen. Estrogen can also increase the expression of Bcl-2 in osteoblast. All of these may play a role in inhibiting osteoblast apoptosis induced with serum hungry.
出处
《中国康复理论与实践》
CSCD
2006年第11期960-962,共3页
Chinese Journal of Rehabilitation Theory and Practice
