傅里叶变换红外光谱法直接测定并结合主成分分析鉴定白芍及其伪品的研究

Direct Determination by FTIR Spectroscopy Combined with PCA for the Identification of Paeonia lactiflora Pall. and Their Confusable Varieties

目的采用傅里叶变换红外光谱(FTIR)法并借助OMNI采样器直接测定白芍及其伪品的FTIR。方法采用FTIR直接测定法获得样品的红外光谱,以样品木质部作为比较标准,分别选取36组具有代表性吸收峰的吸光度数据为基础,以样品为分析对象进行主成分分析。应用主成分析法比较正伪品之间的差异程度。结果FTIR的主成分分析在反映同属不同种植物化学组成差异程度上具有应用价值。结论该方法能直接、快速、准确地测定样品的FTIR图谱。

Objective To identify Paeonia lactiflora Pall. and its confusable varieties by Fourier transform infrared （FTIR） spectroscopy assisted with OMNI sampler. Methods IR was obtained by FTIR Spectroscopy with direct mensuration , principal component analysis （PCA） was progressed by taking xylem as comparing standard, selecting thirty-six groups representative absorbency data as a basis, and taking samples as analysis objective. The degree of difference between Paeonia lactiflora Pall. and its confusable varieties was compared by principal factors analysis. Results Principal component analysis of FTIR Spectroscopy exhibited practical value on reflecting the degree of difference in chemical compositions among different species from the same genera. Conclusion This is a direct, quick and accurate method to obtain the sample FTIR atlas.