摘要
用阳极极化法在碱性溶液中活化玻碳电极，研究了尿酸（UA）在活化玻碳电极（AGCE）上的电化学行为，并提出一种利用微分脉冲伏安技术测定全血中尿酸的电化学分析方法。在0．1mol／L的乙酸缓冲溶液中（pH5．0），以0．1mol／L KCl作为支持电解质，尿酸在AGCE上于0．484V处产生一个灵敏的氧化峰。微分脉冲伏安法测定其氧化峰电流与UA的浓度在5．0×10^-6～2．0×10^-4mol／L范围内呈良好的线性关系，相关系数为0．9989，检出限为1．0×10^-6mol／L。该方法操作简便，重现性较好，能在抗坏血酸存在下同时测定UA。用于人血中UA的测定。
An activated glassy carbon electrode （AGCE） was generated by anodic polarization in the alkali solution. The electrochemical behavior of uric acid （UA） was investigated at the AGCE by cyclic voltammetry （CV） and linear sweep vohammetry （LSV）. A novel method was proposed for the determination of UA in human whole blood by differential pulse voltammetry （DPV）. A sensitive oxidation peak was observed at 0.484 V （ vs. SCE） in 0.1 mol/L HAc -NaAc buffer solution （pH 5.0） with 0.1 mol/L potassium chloride as supporting electrolyte. The peak current is well-proportional to the concentration of UA over the rang from 5.0 × 10^-6 to 2.0× 10^-4 mol/L by DPV, with the correlation coefficient of 0. 9989. The activated glassy carbon electrode is easy to be prepared with good reproducibility and it can be used for the determination of UA in the presence of ascorbic acid. The method was employed for measurement of UA in human whole blood with satisfactory results.
出处
《分析试验室》
CAS
CSCD
北大核心
2007年第1期27-31,共5页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金(29975025)项目资助
关键词
活化玻碳电极
尿酸
血
循环伏安法
微分脉冲伏安法
Activated glassy carbon electrode
Uric acid
Blood
Cyclic voltammetry
Differential pulse vohammetry
