手术前后血管内皮生长因子、血小板活化因子水平与非小细胞肺癌的关系

The Relations between the Levels of VEGF,Activation Markers of Platelets around Operation and Their Relationship with Lung Cancer

目的:探讨非小细胞肺癌患者手术前、后血清血管内皮生长因子(VEGF)、外周血血小板活化标志物的动态变化规律及其与非小细胞肺癌(NSCLC)的关系。方法:用ELISA法检测120例非小细胞肺癌患者手术前、后血清VEGF水平和60例健康体检者VEGF水平;同时用流式细胞术(FCM)检测其外周血血小板活化标志物水平。结果:①NSCLC患者手术前、后VEGF水平和血小板活化标志物水平均显著高于正常对照组(P<0．01);②NSCLC患者手术后第7天血清VEGF水平显著高于术前和术后第1天水平(P<0．01);而血小板活化标志物水平则相反;③NSCLC患者血清VEGF和外周血血小板活化标志物水平与有无淋巴结转移和TNM分期有密切关系(P<0．05)。结论:NSCLC患者血清中VEGF和外周血血小板活化标志物水平都升高,且与NSCLC的分期有密切关系,它们可作为动态检测NSCLC患者病情进展、判断预后的参考指标。

Objective： To study the relationship between the serum levels of VEGF, the levels of activation markers of platelets in peripheral blood around operation in NSCLC patients. Methods： The serum levels of VEGF were detected in 120 patients with NSCLC and 60 healthy persons by ELISA method. At the same time their levels of activation markers of platelets in peripheral blood are examined by FCM method. Result： （1） The serum levels of VEGF and the levels of activation markers of platelets in peripheral blood in NSCLC patients were significantly higher than those healthy people （P〈0.01）. （2） The serum levels of VEGF in NSCLC patients on the seventh postoperative day was significantly higher than that before operation and on the first postoperative day （P〈0.01） but the levels of activation markers of platelets in peripheral blood is opposite. （3） The serum levels of VEGF and the levels of activation markers of platelets in peripheral blood in NSCLC patients were closely related to the lymph node status and TNM stages. Conclusion： Elevation of serum levels of VEGF and the levels of activation markers of platelets in peripheral blood exist in NSCLC patients and they play an important role in tumor growth and lymphatic metastasis, which may be two predictors for prognosis.