巨龙竹种子、小穗外植体愈伤组织的诱导培养

A Study on Callus Induction Culture of Dendrocalamus sinicus

通过对巨龙竹种子和小穗两种外植体愈伤组织初导培养基和增生培养基的筛选及防褐化处理试验，结果表明，巨龙竹种子和小穗初导培养基最优组合为3／4MS＋3mg／L2，4-D＋0．3mg／LKT＋25e／L，MS＋3mg／L2，4-D＋0-3nlg／LKT＋25g／L；其种子和小穗的增生培养基最优组合分别为MS＋（3～5mg／L）2．4-D＋0．3ms／LKT、MS＋3ms／L2，4-D＋0．3ms／LKT；在特定培养条件下，对褐变有一定程度的控制。

Experiment of callus induction culture of Dendrocalamus sinicus including medium screening and brownprevented was conducted by using seed and small bamboo spike as explants. The experimental results showed that the optimum media for callus induction of seeds and small bamboo spike were 3/4 MS＋ （3 ～ 5 rag/L） 2,4 -D ＋ 0. 3 mg/L KT and MS ＋ 3 mg/L 2,4 - D ＋ 0. 3 mg/L KT respectively, the rate of callus induction achieved 100 %. The optimum multiplication media were MS ＋ （3 ～ 5 mg/L） 2,4 -D ＋ 0.3 mg/L KT and MS ＋ 3 mg/L 2,4 - D ＋ 0. 3 mg/L KT respectively. The experimental results also indicated that culture under dark condition, the brown phenomenon could be somewhat prevented.