高效液相色谱法测定复方卡托普利片剂的含量

HPLC determination of compound captopril tablets

目的用高效液相色谱法测定复方卡托普利片剂中卡托普利和氢氧噻嗪的含量。方法采用高效液相色谱法，使用Hypersil CN（150mm×4．6mm，5μm）色谱柱，以甲醇-四氢呋喃-水（10：10：80，V／V，磷酸调pH=2．86）为流动相。流速1．0mL·min^-1，检测波长220nm。结果卡托普利在50-250μg·mL^-1线性较好，其线性方程为C=1．338×10^-4A-2．563（n=5，r=0．9999）；氢氯噻嗪在26～130μg·mL^-1线性较好，其线性方程为C=1．804×10^-5A-1．997（n=5，r=0．9999）。卡托普利的平均回收率为100．6％，RSD=0．2％（n=6），氢氯噻嗪的平均回收率为100．6％，RSD=0．3％（n=6）。结论该方法快速简捷、可靠、准确度高、重现性好，可同时测定复方制剂中2种成分。

OBJECTIVE To establish an HPLC method for the determination of captopril and hydrochlorothiazide in their compound captopril tablets. METHODS The HPLC separation was carried out on a Hypersil CN （150 min× 4. 6 mm, 5 μm） column with methanol-tetrahydrofuran-water （adjusted to pH= 2. 86 with phosphoric acid） （10 ： 10 ： 80） as the mobile phase, detected at 220 nm, flow rates 1.0 mL · min^-1. RESULTS Two good linearities were demonstrated by linear equation C=1. 338X10-4A=2. 563 （n=5, r= 0. 999 9） for captopril and C=1. 804X10 SA -1. 997 （n=5, r = 0. 999 9） for hydrochlorothiazide. The average recoveries of captopril and hydrochlorothiazide were 100.6%, RSD=0.2% （n=6）, and 100. 6%, RSD=0. 3% （n=6）. CONCLUSIONS The method is simple, reliable and accurate for the simultaneous determination of captopril and hydrochlorothiazide.