高效液相色谱法测定七维牛磺酸口服溶液中维生素C的含量

Determination of vitamin C in seven vitamins and taurine oral solution by HPLC

目的采用高效液相色谱法测定七维牛磺酸口服溶液中维生素C的含量。方法采用Diamonsil ODS C18柱（150mm×4．6mm），5μm）；流动相为0．01mol·L^-1磷酸二氢钠溶液（pH值为2．4）～乙腈（99：1）；检测波长为244nm。结果线性范围2．689～43．03μg，r=0．9997，平均回收率100．0％，RSD=2．0％（n=9）。结论该方法简便，结果准确可靠，重现性好。

OBJECTIVE To establish an HPLC method for the determination of vitamin C in 7 vitamins and taurine oralsolution. METHODS A Diarnonsil ODS C18 （150 min×4. 6 Mm, 5 μm） column was used with 0. 01 mol· L^-1 sodium dihydrogen phosphate （pH 2.4） -aeetonitrile （99 ,1） as the mobile phase; content was detected at 244 nm. RESULTS The linear range was 2. 689～43.03μg, and r=0. 999 7. The average recovery rate was 100. 0%, RSD=2.0% （n=9）. OONCLUSIONS The method is simple, rapid, accurate and reproducible.