复方中药对酒精性肝病大鼠核因子κB的影响

Effect of compound Chinese drug on the expression of nuclear factor kappa-B in rats with alcoholic liver disease

目的:探讨复方中药对酒精性肝病核因子κB(NF-κB)的影响。方法:63只大鼠随机分为正常组、酒精组、中药组。酒精组及中药组分别用酒精和中药灌胃,在4、8、12周末分别处死3组大鼠,观察肝脏病理改变及肝组织中NF-κB的表达。结果:随着酒精性肝病的发展,酒精组大鼠肝细胞坏死增多,炎细胞浸润成团,细胞胞浆NF-κB的着色逐渐增强。而复方中药组病理改变和NF-κB的表达明显减弱。结论:NF-κB表达的强弱反映了酒精性肝损害的程度。复方中药能防止酒精性肝损害,有效地抑制NF-κB的表达。

Objective： To explore the effect of compound Chinese drug on the expression of nuclear factor kappa-B （NF-κB） in rats with alcoholic liver disease. Methods：Sixty-three rats were randomly divided into normal group, alcohol group, and Chinese drug group. The rats in alcohol group and Chinese drug group were lavaged with alcohol and compound Chinese drug, respectively. The rats in each group were killed at the end of week 4, 8, and 12. The pathological changes of liver and expression of NF-κB were determined. Rsesults：In alcohol group, the. hepatocyte necrosis increased with the development of the alcoholic liver disease; the inflammatory cells infiltrated and clustered; the expression of NF-κB increased gradually. The pathological changes and expression of NF-κB in compound Clfinese drug appeared later and lighter than those in alcohol group. Conclusion：The expression of NF-κB reflects the level of alcoholic liver damage. Compound Chinese drug can effectively inhibit the expression of NF-κB and prevent the alcoholic liver damage.