5-羟色胺在肠易激综合征中作用的研究

Role of 5-hydroxytryptamine in patients with irritable bowel syndrome

目的:探讨5-羟色胺(5-HT)在肠易激综合征发病中作用以及与其临床症状的相关性。方法:免疫组化方法染色,兔抗人5-HT多克隆抗体测定5-HT阳性细胞,计数阳性细胞数量并进行分析。结果:肠易激综合征直肠-乙状结肠交界部5-HT阳性细胞明显高于正常对照组(P<0.05),腹泻型肠易激综合征增加更明显(P<0.01)。结论:5-羟色胺对结肠的运动、分泌以及感觉等有调节作用。

Objective： To explore the role of 5-hydroxytryptamine （5-HT） in patients with irritable bowel syndrome （IBS） and the relationship between 5-HT and clinical symptoms. Methods：Samples obtained from colonoscopy were stained immunohistochemieally with rabbit anti-human 5-HT polyclonal antibody. 5-HT positive cells were counted, and quantitative analyses of the numhcr of 5-HT positive cells were performed. Results：The number of 5-HT positive cells in reeto-sigmoid junction in patients with IBS, especially in patients with diarrhea-predominant IBS, wassignlficantly higher than that in normal controls （ P 〈0.05, P 〈0. 01 ）. Concluslon：5- HT may play a regulatory role in the movement, secretion, and sensation of colon.