肿瘤坏死因子相关凋亡诱导配体诱导骨肉瘤细胞凋亡的研究

Experimental study on apoptosis of osteosarcoma cells induced by TNF-related apoptosis-inducing ligand

目的:研究肿瘤坏死因子相关凋亡诱导配体(TRAIL)对骨肉瘤OS-732细胞的诱导凋亡作用,探寻骨肉瘤临床化疗的新方案。方法:将TRAIL应用于体外培养的骨肉瘤OS-732细胞,采用MTT法检测细胞毒性作用,倒置相差显微镜及荧光染色方法观察肿瘤细胞形态改变,扫描及透射电镜在亚细胞形态上证实凋亡细胞。结果:不同浓度TRAIL作用下,细胞抑制率间差别十分显著(P<0.01)。超微结构观察显示骨肉瘤细胞凋亡。结论:TRAIL诱导骨肉瘤细胞凋亡,且具有浓度依赖性。

Objective： To study the role of TNF-related apoptosis-inducing ligand （TRAIL） in the apoptosis of osteosarcoma cell line （OS-732） and explore the new chemotherapeutic strategy. Methods： TRAIL was administered to OS-732 cells cultured in vitro, and the inhibition rate of OS-732 cells was measured by using MTT assay. The cellular ultrastructure was observed under inverted phasecontrast microscope, fluorescence microscope, and scanning and transmission electron microscope. Results： There was significant difference in the inhibition rate among OS-732 cells under different concentrations of TRAIL 24 hours after administration of TRAIL （ P 〈 0.01 ）. The changes of cellular ultrastructure showed TRAIL induced the apoptosis of OS-732 cells. Conclusion： TRAIL could induce the apoptosis of osteosarcoma ceils in a dose-dependent manner.