摘要
本研究应用血凝抑制试验(H I),2004年初H 5亚型禽流感在中国内地发生前后,于湖北、广西二疫区市县的生态系中,自健康的鹰、鸽子、野鸭和鹌鹑中采集了54份血清,对其进行了血清学检测,并将获得的血清学检测数据进行了统计学分析。分析结果显示,在总共采集的54份血清样品中,抗H 6亚型禽流感病毒(H 6)抗体、抗H 9亚型禽流感病毒(H 9)抗体、抗H 5亚型禽流感病毒(H 5)抗体阳性率分别为64.8%、75.9%、29.6%。推断,这一时期,H 6亚型禽流感病毒(H 6)、H 9亚型禽流感病毒(H 9)和H 5亚型禽流感病毒(H 5)共循环于该生态系中,其中H 9、H 6亚型禽流感病毒长期、稳定循环于该生态系中,为该生态系禽流感主要流行血清型。血凝抑制试验检测结果发现,H 5亚型禽流感病毒抗体滴度较低,最高仅为640×,其阳性率为29.6%,推断其可能为新近传入的病毒或新近重组变异出现的新病毒,可能为与该次禽流感暴发相关的新病毒,这一推断还有待于通过对病毒基因序列的分析而做进一步验证。
Hemagglutinin-Inhibition test is used in this study to monitor the 54 serum samples from healthy hawks, pigeons and quails in the ecosystem in Hubei and Guangxi province, where there were outbreaks of H5 subtype HPAI in 2004. In addition, the data are statistically analyzed. The results show that-the positive rates of anti, H9 subtype, H6 subtype aiv and H5 subtype aiv antibodies is 64.8% .75.9% and 29. 6% respectively. According to these results, we drew the conclusion that H9 subtype aiv, H6 subtype aiv and H5 subtype aiv circulated around this ecosystem. And H9 subtype aiv and H6 subtype aiv circulated stably and chronically in this ecosystem and are the leading prevalent serotype. On the other hand, the positive rate of anti H5 subtype aiv antibodies is 29. 6%, the highest titer of antibodies of that is 640 ×. It is impossible that the H5 subtype a iv is newly transmitted into the ecosystem or a new recombinant strain of virus. And it is correlated to the outbreaks at athis time there, which need to be validated at a molecular level.
出处
《中国兽医杂志》
CAS
北大核心
2006年第12期3-5,共3页
Chinese Journal of Veterinary Medicine
关键词
生态系
抗禽流感病毒抗体
血凝抑制试验
ecosystem
antibody anti air
Hemagglutinin-Inhibition test
statistics