碱性成纤维细胞生长因子对宫内生长受限胎鼠脑保护作用的研究

Protective effect of basic fibroblast growth factor in the brain of fetal rats with intrauterine fetal growth retardation

目的:研究宫内发育阶段的神经系统损伤及其防治,探讨外源性给孕鼠应用碱性成纤维细胞生长因子(bFGF)对宫内生长受限(FGR)胎鼠脑损伤的保护作用。方法:利用子宫动脉钳夹法制作FGR胎鼠模型。将孕鼠分为3组:FGR模型组、假手术对照组和bFGF治疗组。经腹腔给予bFGF4u/g。采用RT-PCR法测定脑组织S100-β及生长相关蛋白43(GAP-43)mRNA的表达。结果:FGR组胎鼠体质量(3.312±0.469)g,明显低于对照组(4.263±1.016)g(P<0.001);bFGF治疗组胎鼠体质量(3.357±0.429)g,较FGR组略有增高。S100-βmRNA的表达,FGR组(1.213±0.273)明显高于对照组(0.989±0.279,P<0.01);bFGF组(1.037±0.390)与对照组较为接近(P>0.05),略低于FGR组(P<0.05)。GAP-43 mRNA的表达,FGR组(1.152±0.222)与对照组(1.307±0.191)相比显著减少(P<0.01);bFGF组减少不明显(1.241±0.214),与对照组相比无差异(P>0.05)。结论:子宫动脉钳夹法复制出的FGR胎鼠脑组织中S100-β及GAP-43 mRNA表达异常可能与胎鼠神经系统损伤有关。外源性给母鼠应用bFGF治疗后可能对胎鼠的神经系统损伤起到一定的保护性作用。

Objective： To study the protective effect of basic fibroblast growth factor （bFGF） on cerebral development of fetal rats with intrauterine fetal growth retardation （FGR）. Methods： FGR rat model was established by clamping the uterine artery. The pregnant rats were divided into 3 groups： FGR group, sham operation group, and bFGF group. The rats in bFGF group were intraperitoneally injected with bFGF of 4 u/g. The expressions of S-100β and growth-associated protein-43 （GAP-43） mRNA were measured by reverse transcfiptase pulymerase chain reaction. Results： The body weight of fetal rats in FGR group （3.312±0.469） and bFGF group （3.357±-0.429） was significantly lower than that in sham operation group （4.263±1.016,P 〈 0.001）. The expression of S-100β mRNA in fetal rat brain in FGR group was significantly higher than that in sham operation group （ 1.213±-0.273 vs. 0.989±0.279, P 〈 0.01 ）, and the expression in bFGF group was slightly lower than that in FGR group （ 1.037±0.390 vs. 1.213±0.273, P 〈 0.05 ）. The expression of GAP-43 mRNA in FGR group was significantly lower than that in sham operation group （1.152±0.222 vs. 1.307±0.191, P 〈 0.05）. No significant difference in GAP-43 mRNA expression was found between bFGF group and sham operation group （1.241±0.214 vs. 1.307±0.191, P 〉 0.05 ）. Conchusion： Intrauterine ischemia could increase the expression of S-100β and decrease the expression of GAP-43, which indirectly suggests the brain injury of fetal rats with FGR. The administration of bFGF has protective effect on brain injury in fetal rats with FGR.