血小板活化和内皮细胞损害对大肠癌影响的研究

Effect of platelet activation and endothelial cell injury on colorectal cancer

目的:研究血小板活化和内皮细胞损害对大肠癌病情和预后的影响。方法:采用放射免疫法和酶联免疫法测定55例大肠癌和40例正常人血浆血栓烷B2(TXB2)、11-去氢-血栓烷B2(DH-TXB2)、血小板α-颗粒膜蛋白(GMP-140)、血管性血友病因子(vWF)、血栓调节蛋白(TM)、血清纤维结合蛋白(FN)和环磷酸鸟苷(cGMP)的含量。结果:大肠癌TXB2、DH-TXB2、GMP-140、vWF、TM和cGMP升高,FN下降。大肠癌cGMP与TXB2、DH-TXB2、GMP-140、TM之间均呈正相关。大肠癌已转移FN下降,其余指标升高;手术后FN升高,其余指标下降。结论:大肠癌患者存在血小板活化和内皮细胞损害,二者影响大肠癌的转移和预后。

Objective： To study the effect of platelet acfivalion and endothelial cell injury on the metastasis and prognosis of colorectal cancer. Methods： The levels of thromboxane B2 （TXB2）,11-dehydro-thromboxane B2 （DH-TXB2）,granulomembrane protein 140 （GMP- 140）,yon Willebrand factor （vWF）,thrombomodulin （TM）,fibronectin （FN）,and cyclic guanosine monophosphate （cGMP） were determined by radioimmunological method and enzyme-linked immtmosorbent assay in 55 patients with colorectal cancer and 40 normal controls. Results： In patients with colorectal cancer,the levels of TXB2,DH-TXB2,GMP-140,vWF,TM,and cGMP increased,and the level of FN decreased;the level of cGMP was positively related to the levels of TXB2,DH-TXB2,GMP-140,and TM. FN level decreased in patients with metastatic colorectal cancer,while the other indexes increased. FN level increased after operation,and the other indexes decreased. Conclusion：The activation of platelet and injury of epithelial cell exist in patients with colorectal cancer, and they may affect the metastasis and prognosis of colorectal cancer.