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蚕豆离体培养繁殖植株对高温及紫外线胁迫的反应

RESPONSE OF in vitro PROPAGATED PLANTLETS OF Vicia faba TO HIGH TEMPERATURE AND ULTRAVIOLET STRESS
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摘要 将蚕豆种子无菌苗(对照)和离体繁殖3代无菌苗作为供试材料,观察它们在高温和紫外线胁迫下的生长和根尖细胞有丝分裂的情况。试验结果表明,供试植株在高温和紫外线胁迫下在外观上表现叶片枯黄、植株死亡,在染色体水平上发生了畸变,紫外线胁迫造成的畸变程度大于高温胁迫,3代克隆苗的畸变程度大于种子苗。 Using propagated-plantlets from the 3^rd generation of Viciafaba as materials, the effects of heat and ultraviolet stress on growth and the mitosis of root-tip ceils of plantlets were studied. The results showed that the frequency of the plantlets with yellow leaf and dead plantlets and chromosomal aberrations were increased by use of both stress compared with CK, the effects of ultraviolet stress were stronger than that of heat stress. The frequency of chromosomal aberrations of plantlets from the 3^rd generation was higher than that of plantlets germinated from seeds.
出处 《核农学报》 CAS CSCD 北大核心 2006年第6期469-472,共4页 Journal of Nuclear Agricultural Sciences
关键词 蚕豆 快繁 高温和紫外线胁迫 染色体畸变 Vicia faba rapid propagation heat and ultraviolet stress chromosomal aberration
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