HPLC测定四川及青海地区獐牙菜植物中6种主要成分的含量

Simultaneous determination of six main constituents in Swertia of Qinghai province and Sichuan province by HPLC

目的：建立反相高效液相色谱法同时测定四川及青海地区部分獐牙菜中獐牙菜苦苷、龙胆苦苷、芒果苷、当药醇苷、异荭草苷、1,8-二羟基-3-甲氧基咄酮的含量。方法：采用RP-HPLC,使用Kromasil C_(18)(4．6mm×2．50 mm,5μm)柱；流动相甲醇-水(含0．02%磷酸)；梯度洗脱程序为0～50 min,甲醇的体积分数(下同)由20%上升至80%；50-55 min由80%增至100%,55-60 min为100%,流速1 mL·min(-1),检测波长254 nm；柱温35℃。结果：6种成分均达到基线分离,线性良好。结论：该方法快速、准确、重复性好,为该类药材的入药提供了理论依据。

Objective： To establish a quantitative method of simultaneously determination of swertiamarin, gentiopicroside, mangiferin, swertianolin, isoorientin, 1,8-drihydroxy-3-methoxy-xthanone in Swertia from Qinghai province and Siehuan province by HPLC. Method： The samples were separated on the column of Kromasil C18（4. 6 mm ×250 mm,5 μm） which eluted with methanol and water（content 0. 02% phosphoric acid）. The ratio of methanol increased from 20% to 80% during 20-50 min,and from 80% to 100% during 50-60 min, with detected wavelength 254 nm,flow rate at 1 mL · min^-1 ,column temperature 35 ℃. Result： Six compounds were base-isolated, the linear ranges of swertiamarin, gentiopieroside, mangiferin ,4-swertianolin, 5-isoorientin, 1,8-drihydroxy- 3-methoxy-xthanone were excellent. Conclusion： The method was rapid and precise, and can be use for controlling medicinal materials quality.