摘要
目的:对红藻扇形叉枝藻Gymnogongrus flabelliformis进行化学成分研究。方法:利用正相和反相硅胶柱色谱、Sephadex LH-20凝胶柱色谱和反相HPLC等手段进行分离纯化,借助MS,1D和2D NMR等波谱方法鉴定化合物结构;通过MTT法对分离鉴定的单体化合物在人肺癌细胞株(A549)、人肝癌细胞株(Bel 7402)、人胃癌细胞株(BGC 823)、人结肠癌细胞株(HCT-8)和人卵巢癌细胞株(A2780)上进行肿瘤细胞毒活性筛选。结果:从红藻扇形叉枝藻中分离得到5个降碳倍半萜类化合物,分别鉴定为(3S,6R,7E)-(+)-3-hydroxyl-4,7-megastigmadien-9- one(1),(3S,5R,6S,7E)-(-)-3-hydroxy-5,6-epoxy-7-megastig-mene-9-one(2),(3S,5S,6R,7E)-(+)3-hydroxy-5,6- epoxy-7-megastigmene-9-one(3),dehydrovomifoliol(4)和(3R)-(-)-4-[(2R,4S)-4-acetoxy-2-hydroxy-2,6,6-trimethyl- cyclohexylidene]-3-buten-2-one(5)。结论:以上化合物均为首次从该属海藻中分离得到,其中化合物1为新天然产物。细胞毒活性试验结果显示所有化合物在10μg·mL^(-1)均无明显细胞毒活性。
Objective: To investigate the chemical constituents of the red alga GymnogongrusflabeUiformis Harv. Method: Compounds were isolated by normal phase silica gel and Sephadex LH -20 gel column chromatography and reverse phase HPLC. Their structures were elucidated by spectroscopic methods including MS, ^1 H-NMR,^13C-NMR. Cytotoxicity of the compounds was screened by using standard MTT method. Result: Five compounds were isolated from G. flabeUiformis, their structures were identified as(3S, 6R, 7E) -( + ) -3-hydroxyl-4, 7-mega-stigmadien-9-one ( 1 ), (3S, 5R, 6S, 7E) -( - ) -3-hydroxy-5, 6-epoxy-7-megastigmene-9-one (2), (3S, 5S, 6R, 7E)-( + )3-hydroxy-5, 6-epoxy-7-megastigmene-9-one (3), dehydrovomifoliol (4), (3R)-( - )-4-[ (2R, 4S)-4- aeetoxy-2-hydroxy-2, 6, 6-trimethyleyelohexylidene]-3-buten-2-one (5). Conclusion: All of the compounds were obtained from this species for the first time and compound 1 was a new natural product. These compounds were inactive ( IC50 〉 10 μg · mL^-1 ) in the MTT assay against several human cancer cell lines.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2006年第24期2046-2048,共3页
China Journal of Chinese Materia Medica
基金
教育部"新世纪优秀人才支持计划"
国家自然科学基金重点项目(20432030)