胃癌发病风险与醌氧化还原酶基因多态性的关系

NAD(P)H: quinone oxidoreductase gene polymorphism association with gastric carcinoma

目的：探讨醌氧化还原酶基因多态性与胃癌发病的关系。方法：采用聚合酶链反应-限制性片段长度多态性（PCR-R FLP）分析方法分析了80例胃癌患者与80名健康成人NQ01的基因多态性。结果：醌氧化还原酶基因cDNA609位T等位基因频率在胃癌组和对照组分别为55％和42．5％，两组比较差异有统计学意义，x^2=5．00，P=0．018。基因型分布在胃癌组和对照组之间差异有统计学意义，x^2=7．63，P=0．025。杂舍型（T／C）和突变纯舍子型（T／T）基因型携带者患胃癌的危险性分别是野生型纯合子（C／C）的2．84倍（95％CI：1-2451～6．4780）与3．60倍（95％CI：1．2970～9．9925）。结论：NQ01cDNA609 T等位基因可能是胃癌发生的危险性因素，与胃癌的发生有关。

OBJECTIVE：To explore whether gastric carcinoma was associated with genetic polymorphism of NQO1 gene （NAD（P） H .. guinone oxidoreductase gene） caused by the point mutation of cDNA609 C to T. METHODS： The polymorphism of NQO1 gene was analyzed in 80 patients with gastric carcinoma and 80 unrelated healthy adult individuals by utilizing the polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP） assay. RESULTS.. The frequencies of T allele were 55% and 42.5% in the gastric carcinoma group and the health control group respectively, being significantly different between the two groups （x^2 =5.00, P=0. 018）. The allelic frequency of the mutant T allele was significantly higher in the gastric carcinoma patients as compared with the controls. There was a significant difference in the overall genotypic distribution between the patients and the controls （x^2= 7.63, P=0. 025）. The risk of suffering from gastric carcinoma was increased 2.84 times in the individuals with T/C genotype （95% CI： 1.2451- 6. 478 0） and 3.60 times in the individuals of T/T genotype of NQO1 gene （95% CI：I. 297 0-9. 992 5）. CONCLUSION：The cDNA609 T allele of NQO1 gene may be the risk factor of gastric carcinoma, which can be associated, with genetic susceptibility of gastric carcinoma.