Survivin反义核酸对大肠癌HT-29细胞化疗的影响

Survivin antisense oligodeoxynucleotides effect on the chemotherapy of HT-29 colorectal carcinoma cells

目的:探讨反义寡核苷酸对大肠癌化学治疗敏感性的影响。方法:设计针对Survivin模板序列的经硫代磷酸修饰的反义和正义核苷酸链的寡核苷酸链,导入大肠癌HT-29细胞中,观察其对大肠癌细胞活力、细胞增殖能力的影响以及Survivin反义核酸对大肠癌细胞化疗敏感性的影响。结果:ASODN明显抑制大肠癌细胞的集落形成能力。低浓度5-FU+Survivin反义核酸对HT-29大肠癌细胞的抑制作用明显高于单独低浓度5-FU组和低浓度5-FU+SODN组,其差异有统计学意义,P<0.05。结论:Survivin反义寡核苷酸作用后的大肠癌HT-29细胞株生长受到抑制,Survivin反义寡核苷酸对体外培养的大肠癌细胞化疗有增敏效应。

OBJECTIVE： To investigate the effect of Survivin gene on the growth of human colorectal cancer HT-29 cells and to explore the feasibility of gene therapy in human colorectal carcinoma. METHODS： The antisense oligodeoxynucleotides were complementary to Survivin sequences. The human colorectal cancer HT-29 cell line was treated with ASODNs . The effects of the Survivin-ASODN on the growth of HT-29 and apoptosis were studied by cell colony assay. Low concentration 5-FU was added to study the sensitivity of colorectal cancer cell induced by ASODNs to chemotherapy, RESULTS： The cell proliferation was inhibited effectively by antisense oligodeoxynucleotides to Survivin gene. Compared with the control group,the percentage of the survival cells significantly decreased in the presence of both 5-FU and Survivin ASODNs, P〈0.05. CONCLUSIONS：The antisense oligodeoxynucleotides were capable of inhibiting the proliferation of human colorectal carcinoma HT-29 cells. Combination of Survivin ASODN and 5-FU might improve the treatment outcome of colorectal carcinoma.