山东地区乙型肝炎病毒感染者乙肝病毒基因型和亚型的分布

Distribution of hepatitis B virus genotypes and subgenotypes in Shandong area

目的:了解山东地区乙型肝炎病毒(HBV)基因型和亚型的分布。方法:采用特异性引物巢式PCR法,检测140例HBV感染者基因型和基因亚型。结果:140例标本中,129例(92.1%)可检出基因型,其中B基因型12例(9.3%),C基因型109例(84.5%),B/C混合基因型8例(6.2%),未检出A、D、E、F基因型。在B基因型中,均为Ba基因亚型,未检出Bj亚型;C基因型中,C2基因亚型81例(74.3%),非C1/C2基因亚型28例(25.7%);8例B/C混合基因型中,Ba+C2亚型5例,Ba+非C1/C2基因亚型3例。结论:山东地区HBV感染者中,存在HBV基因B、C、B/C型,其中B型均为Ba亚型,C型中以C2基因亚型为主。

Objective： To investigate the distribution of hepatitis B virus （HBV） genotype and subgenotype in Shandong province. Method： HBV genotypes and subgenotypes were detected in 140 patients with HBV infection by using nested polymerase chain reaction based on genotype and subgenotype specific primers. Results： HBV genotype B, C and B/C were identified in 12, 109 and 8 patients, whereas genotype A, D, E and F were not found. All of the 12 HBV genotype B patients were detected as subgenotype Ba. Of the 109 genotype C patients, 74.3% （81/109） was subgenotype C2 and 25.7% （28/109） was neither C1 nor C2. Of the 8 genotype B/C patients, 5 were subgenotype Ba ＋ C2 and 3 were Ba ＋ neither C1 nor C2. Conclusions： Genotype B, C and B/C have been found in Shandong province, and subgenotype Ba inhabits the genotype B and subgenotype C2 predominates genotype C.