藤梨根提取液抗肺癌作用的实验研究

Radix Actinidiae extract inhibits the proliferation of human lung adenocarcinoma cell line A549: an experimental study

目的:观察藤梨根提取液对肺腺癌A549细胞的生长抑制作用,为其治疗肺癌提供实验依据。方法:采用MTT检测法、集落形成试验观察药物对细胞的作用,用流式细胞术检测细胞凋亡,同时分析药物对细胞周期的影响。采用裸鼠移植瘤模型进行体内实验,并绘制移植瘤生长曲线,计算抑制率。结果:各浓度的藤梨根提取液对肺腺癌A549细胞有较强的抑制效应,呈现出明显的时相性和量-效依赖性,流式细胞仪检测结果显示,藤梨根提取液作用后,G0/G1期细胞数增加,细胞出现凋亡,并随药物浓度增大,作用增强。移植瘤体内试验显示,藤梨根提取液高剂量组(按1 000 mg/kg)和低剂量组(按500 mg/kg)的抑瘤率分别为71.40%和40.35%,治疗组移植瘤生长缓慢,体积明显小于对照组(P<0.01)。结论:藤梨根提取液在体内外对人肺腺癌A549细胞生长均有抑制作用,体外作用与G0/G1期阻滞有关,并有诱导人肺腺癌A549细胞凋亡的作用。

Objective： To study the effect of Radix Aetinidiae extractive on the proliferation of human lung adenecarcinoma cell line A549. Methods： M＇IT assay and colony formation test were used to detect the effect of drug on cells in vitro. Flow cytometry was used to measure the cell apoptosis and cell cycle. In vivo, the tumor growth curve was drawn and the inhibition ratio was calculated in the athymic mouse beating A549 cancer cells treated with Radix Actinidiae extracfive. Results： Radix Actinidiae extractive can inhibit the A549 cell proliferation in time-dependent and dose- dependent manner. Flow cytometry displayed that cells in G0＋ G1 phases increased and the apoptosis was induced after the Radix Actinidiae extractive treatment. The tumor inhibiting rates of high-dose and low-dose group were 71.40% and 40.35%, respectively. The transplanted tumor of treatment group grew slowly and was smaller than that of control group. Conclusion：Radix Actinidiae extractive has anticancer activity on human lung adenocarcinoma cell line A549 and can induce the apoptosis of A549 cells both in vivo and in vitro, and its inhibition in vitro may be related to the blockade of G0 ＋ G1 phases.