胃腺癌中β-连环蛋白、c-myc表达及其临床意义

Detection ofβ-catenin and c-myc expression in gastric adenocarcinoma by tissue microarray and its clinical significance

目的研究胃腺癌和癌旁组织中β-连环蛋白和c-myc的表达及其临床意义。方法收集102例胃腺癌患者的手术切除标本共297份,其中取自癌组织102份,癌旁组织195份(肿瘤边缘2cm以内97份,5cm以外98份)。将标本分别制成282点、156点和156点三块组织芯片,采用免疫组织化学法检测胃组织芯片中β-连环蛋白和c-myc的表达。结果β-连环蛋白和c-myc基因表达在胃黏膜癌变过程中呈上升趋势,从肠上皮化生开始即有明显增高,在胃癌显著高于肠上皮化生(P＜0．0 5,P＜0．001)。β-连环蛋白的异常表达与胃腺癌的分化程度及浸润深度有关(P＜O．05),c-myc表达与胃腺癌的分化程度有关(P＜0．05)。胃腺癌中β-连环蛋白和c-myc表达呈明显正相关(P＜0．05)。结论β-连环蛋白异常表达可能激活靶基因c-myc的表达,并在胃腺癌发生发展中起重要作用。

Objective To study the expression and clinical significance of β-catenin and c-myc in gastric adenocarcinoma and corresponding para cancer gastric mucosa. Methods Two hundreds and ninety-seven samples were collected from 102 cases of gastric adenocarcinoma, including 102 cancerous tissues and 195 para-cancerous tissues. Each sample was made into 282, 156 and 156 dots tissue microarrays. Expressions of β-catenin and c-myc proteins were detected by immunohistochemical staining. Results The expressions of β-catenin and c-myc were increased gradually in the process of gastric carcinogenesis. The rates of β-catenin and c-myc expression were higher in carcinoma than that in intesti nal metaplasia（ P 〈 0.05, P 〈 0. 001）. The aberrant expression of β-catenin was closely related to the depth of invasion（ P 〈 0.05）, and the expression of c-myc was related to histological grade（ P 〈 0.05）. The aberrant expression of β-catenin was significantly correlated with the expression of c-myc in gastric adenocarcinoma（ P 〈 0.05）. Conclusions The aberrant expression of β-catenin may activate the expression of target gene c-myc, which plays a pivotal role in the carcinogenesis and progression of gastric adenocarcinoma.