抗转移多肽对人胃癌腹膜高转移细胞侵袭转移的抑制作用

Inhibitory effects of synthetic PⅢpeptide on invasion and peritoneal metastasis of gastric carcinoma with high peritoneal metastasis potential cell line

目的研究多肽PⅢ对人胃癌腹膜高转移细胞系GC9811-P腹膜转移的作用。方法利用体外细胞基质粘附和体外细胞侵袭实验,分别检测多肽PⅢ对GC9811-P细胞粘附及侵袭能力。体内实验采用胃浆膜下裸鼠原位接种转移模型,观察多肽PⅢ对胃癌细胞GC9811-P腹膜转移能力的影响。裸鼠分多肽PⅢ治疗组、0．9%氯化钠溶液对照组各12只,荷瘤鼠衰竭处死后观察腹膜转移率、转移灶数目及原发瘤质量。结果40μg多肽PⅢ孵育2h时粘附抑制率达86．3%,多肽PⅢ与GC9811-P细胞共同孵育对层粘连蛋白粘附的抑制作用呈时间依赖性。多肽PⅢ与GC9811-P细胞共孵育48h后侵袭抑制率达81．4%。将肿瘤细胞原位接种于裸鼠后给予多肽PⅢ治疗,与对照组比较腹膜转移灶的数目分别为(3．2±6．5)个和(26．3±5．2)个,腹膜转移率明显下降(P＜0．01)；而原发瘤质量分别为(1．9±1．2)g和(2．1±1．0)g,两者间差异无统计学意义(P＞0．05)。结论多肽PⅢ明显抑制人胃癌腹膜高转移细胞系GC9811-P粘附、侵袭和腹膜转移作用。

Objective To evaluate the potential utility of PⅢ peptide in anti-peritoneum metastasis in gastric carcinoma cells with high peritoneal metastasis potential（GC9811 -P）. Methods The adhesion and invasion inhibitory effects of PⅢ peptide on GC9811-P cells were detected by in vitro matrix adhesion and cell invasion experiments. By using nude mice metastatic model of human gastric cancer, the effects of PⅢ peptide on peritoneal metastasis of gastric cancer GC9811-P were evaluated. Mice were randomly divided into the experimental（GC9811 P ＋ peptide PⅢ group） and control groups（GC9811-P ＋0.9% NaCl solution group）, 12 mice in each group. At the exhaustion time after inoculation, mice were sacrificed to observe the incidence of peritoneal metastasis, the number of the metastasis loci and the volume of primary tumor. Results Two hours after 40 μg Pill peptide incubation, the adhesion inhibitory rate reached 86.3 %. The adhesion inhibitory effects were in a time dependent manner, The invasion inhibitory effects became apparent（81.4%） 48 hours after P Ⅲ peptide insult. After the GC9811- P cells were orthotopic implanted in nude mice and treated with PⅢ peptide, the number of peritoneal metastatic nodes were significantly reduced as compared with control group（3.2±6.5 vs. 26.3±5.2） （ P 〈 0.01 1. But the mass of primary tumor were （ 1.9 ±1.2） g in PⅢ peptide treated group and （2.1 ± 1.0） g in the control group, no difference was noted between two groups（ P 〉 0 05 ）. Conclusion PⅢ peptide can markedly inhibit the adhesion, invasion and peritoneal metastasis of gastric carcinoma cell line CA29811 -P with high peritoneal metastasis potential.