异丙酚对N-甲基-D-天冬氨酸诱发大鼠海马神经元内游离钙离子浓度的影响

Effect of propofol on the changes in intraceHular free calcium ion concentration in cultured hippocampal neurons induced by N-methyl-D-aspartate

目的观察异丙酚对N-甲基-D-天冬氨酸(NMDA)诱发大鼠海马神经元内游离钙离子浓度([Ca^(2+)]_i)的影响。方法取当天新生的Wistar大鼠海马神经元,培养12 d随机分为5组,对照组(C组)、NMDA组、异丙酚10μmol／L+NMDA组(P1组)、异丙酚100μmol／L+NMDA组(P2组)、异丙酚400μmol／L+NMDA组(P3组)。NMDA组培养液中加入NMDA至终浓度20μmol／L,P1组、P2组及P3组加入NMDA前即刻分别加入异丙酚至终浓度为10、100、400μmol／L,加入异丙酚后11 min用激光共轭聚焦显微技术测定[Ca^(2+)]_i。结果NMDA可诱发海马神经元[Ca^(2+)]_i升高,预先加入异丙酚100、400μmol／L可抑制这种改变,异丙酚10μmol／L对NDMA诱发的上述改变无影响。结论高浓度异丙酚可抑制NMDA诱发的大鼠海马神经元细胞[Ca^(2+)]_i的升高,这可能是其神经保护作用的机制之一。

Objective To investigate the effect of different concentrations of propefol on the changes in the intracellular free calcium ion concentration （[Ca^2＋ ]i） in the cultured rat hippocampal neurons induced by N- methyt-D-aspartate （NMDA）.Methods Hippocampal neurons were isolated from newborn Wistar rats and cultured for 12 days. The neurons were then randomly divided into 5 groups： group Ⅰcontrol; group Ⅱ NMDA; group Ⅲ, Ⅳ, Ⅴ propofol 10,100 or 400 μmol· L^- 1 ＋ NMDA. In group Ⅱ NMDA was added to the culture media and the final concentration of NMDA reached 20 μmol· L^- 1 . In group Ⅲ , Ⅳand Ⅴpropofol was added to the culture media before NMDA and the final concentrations of propofol reached 10, 100 and 400 μmol ·L^-1 respectively. Confocal laser scanning microscopy was used to measure the [ Ca^2＋ 11 of hippecampal neurons 11 min after propofol was added. Results NMDA significantly increased the [Ca^2＋ ]i of hippecarapal neurons. Pretreatment with propefol 100 or 400 μmol ·L^-1 could significantly inhibit the increase in [Ca^2＋ ]i induced by NMDA, but propefol 10 μmol ·L^-1 did not have the inhibitory action. Conclusion Higher concentrations of propefol can significantly inhibit the increase in [ Ca^2＋ ]i of hippecampal neurons induced by NMDA. This may be one of the mechanisms that contribute to the neuroprotection provided by propofol.