成人视网膜神经细胞的原代培养和初步鉴定

Primary cultivation and initial identification of adult human retinal neural cell

目的建立成人视网膜神经细胞培养系统，为深入研究视网膜细胞及观察药物作用提供基础。方法采用组织块培养法，以含100g·L^-1 FBS的IMDM作为细胞培养液，培养成人视网膜细胞，对培养7—10d的细胞进行初步细胞鉴定。结果体外培养成人视网膜细胞原代培养可达80d左右，早期培养的细胞中视网膜神经细胞占65％左右，其中可见多数带有外节段的感光细胞。结论这种培养方法提供的视网膜细胞早期主要以神经细胞为主，可用于视网膜神经细胞的药物作用研究。

Objective To establish a culture system in vitro of adult human retinal neural cells,and provide a basis for further study on retinal neural cells and drug effect. Methods With Tissue Explants methods and IMDM （including 100 g · L^-1 FBS） as cell culture fluid, the adult human retinal cells were cultttred. After 7 - 10 days cultivation, the retinal cells were initially identified by neuron-specific enolase,glial fibrillary acidic protein, S100 and cytokeratin. Resuits Approximately 65% of these cells were retinal neural cells,among which photoreceptor cells with lateral segment were observed mainly. After being cultured in vitro for 80 days,the retinal neural cells died away gradually. Conclusion The retinal cells cultured by this method are mainly neural cells,which can be used as retinal neural cell models for drug research.