期刊文献+

RNA沉默技术及其在植物中的应用 被引量:10

RNA silencing and its application in plant
下载PDF
导出
摘要 RNA沉默是广泛存在于生物中的一种古老现象,是生物抵抗异常DNA的一种保护机制,同时在生物生长发育过程中扮演着基因表达调控的角色。文章对RNA沉默研究中的几个热点问题进行了介绍,按RNA沉默持续时间的不同对其在植物中应用的方法进行了分析,并重点论述了RNA沉默在植物功能基因组研究、作物品质改良以及抗病毒植物培育等方面的应用及其发展前景。 RNA silencing is an ancient phenomenon commonly existed in organisms, which acts as the mechanism of serf-protection in defending abnormal DNA and as the gene expression regulator during the growth and development of organisms. The hotspots about RNA silencing and the methods of this technique used in plants according to its durational differences were introduced. The current status and prospective of the technique in the areas of plant functional genomics studies, improvement of crop quality, and plant breeding for virus resistance were reviewed.
出处 《遗传》 CAS CSCD 北大核心 2007年第1期22-28,共7页 Hereditas(Beijing)
基金 江西省科技厅攻关项目(编号:赣科技字2000-8号) 江西省教育厅重大项目(编号:赣教技字2005-47号)资助~~
关键词 RNA沉默 RNA干扰 功能基因组 作物品质改良 抗病毒植物 RNA silencing RNA interference functional genomics crop quality improvement virus resistant plants
  • 相关文献

参考文献46

  • 1Napoli C, Lcmieux C, Jorgensen R. Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous gene in trans.Plant Cell, 1990, 2(4): 279--289.
  • 2Makarova KS, Grishin NV, Shabalina SA, Wolf YI,Koonin EV. A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of action.Biol Direct, 2006, 1(1): e7.
  • 3Bartel DE MicroRNAs: genomics, biogenesis, mechanism,and function. Cell, 116(2): 281--297.
  • 4Dugas DV, Bartel B. MicroRNA regulation of gene expression in plants. Curr Opin Plant Biol, 2004, 7(5):512--520.
  • 5Miki D, Itoh R, Shimamoto K. RNA silencing of single and multiple members in a gene family of rice. Plant Physiol, 2005, 138(4): 1903-- 1913.
  • 6Chi J T, Chang HY, Wang NN, Chang DS, Dunphy N,Brown PO. Genomewide view of gene silencing by small interfering RNAs. Proc Natl Acad Sci USA, 2003, 100(11):6343 -- 6346.
  • 7Wesley SV, Helliwell CA, Smith NA, Wang MB, Rouse DT, Liu Q, Gooding PS, Singh SP, Abbott D, Stoutjesdijk PA, Robinson SP, Gleave AP, Green ACT, Waterhouse PM.Construct design for efficient, effective and high-throughput gene silencing in plants. Plant J, 2001, 27(6):581--590.
  • 8Mette MF, Aufsatz W, van der Winden J, Matzke MA,Matzke AJ. Transcriptional silencing and promoter methylation triggered by double-stranded RNA. EMBO J,2000, 19(19): 5194--5201.
  • 9Jones L, Hamilton AJ, Voinnet O, Thomas CL, Maule AJ,Baulcombe DC. RNA-DNA interactions and DNA methylation in post-transcriptional gene silencing: Plant Cell,1999, 11(12): 2291 --2301.
  • 10Aufsatz W, Mette MF, van der Winden J, Matzke AJ,Matzke M. RNA-directed DNA methylation in Arabidopsis. Proc Natl Acad Sci USA, 2002, 99(Suppl. 4): 16499--16506.

二级参考文献38

  • 1Smith A M, Martin C. Starch biosynthesis and the potential for its manipulation. In:Grierson D, eds. Plant Biotechnology ( Vol 3 ). Glasgow, Blackie Academic & Professional,1993,1-54.
  • 2Ball S,Guan H P,James M,Myers A,Keeling P,Mouille G,Buleon A, Colonna P, Preiss J. From glycogen to amylopectin ;a model for the biogenesis of the plant starch granule. Cell, 1996,86:349 - 352.
  • 3Preiss J. Biology and molecular biology of starch synthesis and its regulation. Plant Mol Cell Biol, 1990,7:59 - 114.
  • 4Nelson O E, Rines H W. The enzymatic deficiency in the waxy mutant of maize. Biochem Biophys Res Commun,1962,9:297-300.
  • 5Klosgen R B, Gierl A, Schwarz-Sommer Z, Saedler H. Molecular analysis of the Waxy locus of Zea mays. Mol Gen Genet, 1986,203:237 - 244.
  • 6Rohde W, Becker D, Salamini F. Structural analysis of the Waxy locus from Hordeum vulgare. Nucleic Acids Res,1988,16:7185-7186.
  • 7Wang Z,Wu Z,Xing Y,Zheng F,Guo X,Zhang W, Hong M. Nucleotide sequence of rice Waxy gene. Nucleic Acids Res, 1990,18:5898.
  • 8Van der Leij F R,Visser R G F,Ponstein A S,Jacobsen E,Feenstra W J. Sequence of the structural gene for granulebound starch synthase of potato (Solanum tuberosum L. )and evidence for a single point deletion in the amf allele.Mol Gen Genet, 1991,228:240 - 248.
  • 9Rie T,Midori N, Masayuki I, Ron J O,Susan R W, Ko S.Antisense waxy genes with highly active promoters effectively suppress waxy gene expression in transgenic rice.Plant Cell Physiol, 2000,41 ( 7 ), 881 - 888.
  • 10Liu Q Q,Wang Z Y,Chen X H,Cai X L,Tang S Z,Yu H X,Zhang J L, Hong M M,Gu M H. Stable inheritance of the antisense waxy gene in transgenic rice with reduced amylose level and improved quality. Transgenic Research,2003,12:71-82.

共引文献40

同被引文献172

引证文献10

二级引证文献39

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部