母猪哺乳初期的母性行为与催乳素受体基因多态性关系的初探

Preliminary study on the relationship between sow maternal behaviour during early lactation and polymorphism of PRLR gene

应用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法对民猪和长白猪的催乳素受体(Prolactinreceptor,简称PRLR)基因进行了单核苷酸多态性分析,以研究PRLR基因影响母猪母性行为性状的可能性。结果发现在PRLR基因的cDNA第1620位点处存在NaeⅠ多态性。测序表明,该点存在一个T→C的单碱基突变,属沉默突变。对3种基因型与部分行为性状进行最小二乘分析,结果表明,AB型母猪与其他两种基因型母猪相比,表现出较低的侧卧转为其他姿势的频率以及由母猪结束哺乳的频率(P<0.05),其他行为性状在基因型间不显著。因此,推测等位基因A可能是影响母猪母性行为的不良基因。

PCR-RFLP technique was applied to analyze single nucleotide polymorphisms of PRLR gene in Minpig and Landrace to investigate the possible effect of PRLR gene on sow maternal behaviour. A Nae Ⅰ -RFLP site was detected in the PRLP gene. The single nucleotide polymorphism, a T→C transition at nucleotide 1,620 of the cDNA sequence, was a silent mutation. Least square analysis between the genotypes and the maternal behavioural traits showed that sows with genotype AB had a significantly higher frequency of lateral-lying-to- other-posture trait and percentage of sow-terminated nursing trait than sows with the AA and BB genotypes, although no significant differences were found in other behavioural traits. It is possible that allele A is the unfavorable allele for sow maternal behaviour.