棉铃虫天敌中红侧沟茧蜂Microplitis mediator对不同处理棉花的趋性行为反应

Taxis Responses of Parasitoid Microplitis mediator to Volatiles of Cotton after Different Treatments

采用机械损伤(处理A)、棉铃虫取食为害(处理B)、棉铃虫取食棉花+棉铃虫复合物(处理B+H)、水杨酸诱导(处理C)、机械损伤加棉铃虫口腔分泌物(处理D)5种方式诱导现蕾期棉花植株,并应用Y型嗅觉仪测定处理后棉花对棉铃虫天敌中红侧沟茧蜂搜索寄主行为的影响.处理B、处理B+H和处理C棉花对中红侧沟茧蜂有显著的引诱作用.健康棉株、处理A及处理D棉花对中红侧沟茧蜂没有明显的吸引.中红侧沟茧蜂对处理C、处理B+H以及处理B棉花没有明显的趋性差异.利用气质联用系统对各处理棉花挥发性物质进行定性和定量分析.表明处理B、处理B+H、处理C棉花挥发物种类和含量均比对照棉花多,处理A及处理D棉花与对照棉花挥发物种类和含量差异不显著.处理C、处理B及处理B+H棉花中β-月桂烯、β-蒎烯、α-蒎烯的含量明显增加.处理C棉花柠檬烯的含量发生明显变化.

Taxis responses of Microplitis mediator （Haliday） to the volatiles of cotton plants at squaring stage induced separately by mechanical injury （treatment A ） , damage of cotton bollworm （CBW） Helicoverpa armigera （Hiibner） （treatment B）, steaming with salicylic acid （SA） （treatment C） and treatment A plus oral cavity secretion of CBW （treatment D） were tested in a Y- tube olfactometer. The results showed that treatment B, treatment B plus larval H. armigera, and treatment C had significant attraction for M. mediator, and there were no obvious differences in the attraction of these treatments. Also, there was no obvious attraction of healthy cotton plant, treatment A, treatment D for this parasitoid. The qualitative and quantitative analyses of the cotton volatiles of different treatments were conducted by using GC - MS, and the results indicated that the kinds and contents of cotton volatiles from treatment B, treatment B plus larval CBW and treatment C were more than those of healthy cotton plant, and the differences among those of treatment A and treatment D and healthy cotton plant were not obvious. In comparison with healthy cotton, the contents of β-myrcene, α-pinene and β-pinene were significantly higher, and that of limonene from cotton of treatment C changed obviously. Fig 2, Tab 1, Ref 19