黑灵芝提取物清除DPPH自由基的作用

DPPH Radical Scavenging Effect of Ganoderma atrum

以黑灵芝为研究对象,采用不同方法提取黑灵芝中的有效成分,以BHT和抗坏血酸为对照品,采用DPPH.法研究各种提取物对自由基的清除作用。结果表明:微波萃取法是最适合提取黑灵芝中有效成分的现代方法,不仅产率高,而且速度快,节能;不同溶剂提取物对DPPH自由基清除率强弱依次为95%乙醇>丙酮>水>氯仿,提取物用量与清除能力成量效关系;黑灵芝的丙酮提取物浓度达到1 mg/mL时已超过10 mg/mL BHT的抗氧化效果,相当于0.5 mg/mL浓度抗坏血酸的抗氧化效果。

Using the assay system of DPPH,the antioxidant activities of Ganoderma atrum extracted by different methods were studied and compared with those of BHT and ascerbie acid. The results showed that the microwave-assisted extraction was the best method, which is rapid （about 20 min） ,consumes less solvent and gives higher yield. The scavenging effects on DPPH radical decreased in the order of Ganoderma atrum extracts obtained from 95 % ethanol 〉 acetone 〉 water 〉 chloroform. The antioxidative effect of the extracts is dosage-dependent.The acetone extract of Ganoderma atrum （1 mg/mL） possesses stronger anfioxidant activity than that of BHT （10 mg/mL） and the same of aacorbic acid （0.5 mg/mL）.