RNAi沉默Her-2基因对卵巢癌细胞SKOV3的动物实验研究

Studies the Biological Behavior of Ovarian Cancer Cell Line SKOV3 Silenced Her-2 by RNAi with Animal Experiment

目的观察细胞株SKOV3/si RNA Her-2基因的沉默作用及生物学效应。方法分别将SKOV3/si RNA及对照组SKOV3种植NOD-SCI D小鼠皮下,监测两组瘤体积,3个月后处死NOD-SCI D小鼠,称瘤质量,并采用RT-PCR方法鉴定抑制Her-2表达的效果。结果建立的抑制Her-2基因表达的SKOV3细胞株(SKOV3/si RNA)成瘤力下降。结论动物实验证实RNAi沉默Her-2基因可影响卵巢癌的成瘤力,RNAi技术有可能成为治疗卵巢癌的新途径。

Objective To study the effect of gene silencing Her-2 on SKOV3/siRNA ceils by animal experiment.Methods Cells of SKOV3/siRNA and SKOV3 were transplanted subcutaneously in NOD-SCID mice respectively,observed volume of the two tumor,kiUed mice after 3 monthes and weighed the tumor, RT-PCR was used to assess the effect of Silencing Her-2. Results tumorigenesis of SKOV3/siRNA cell lines in NOD-SCID mice decreased significantly.Conclusion Silencing of Her-2 by RNAi can inhibit tumorigenesis of ovarian cancer with animal experiment and may become a new approach of ovarian cancer therapy.