米非司酮对大鼠在位及异位子宫内膜COX-2表达的影响

Effect of mifepristone on the expression of COX-2 in rat eutopic and ectopic endometrium

目的:探讨米非司酮对大鼠在位及异位子宫内膜环氧合酶-2(COX-2)表达的影响。方法:子宫内膜异位症(endom etriosis,EMS)大鼠随机分为正常对照组、低剂量和高剂量米非司酮组,灌胃4周后,分别取子宫内膜组织用免疫组化法测COX-2的表达。结果:1)不同实验组间在位内膜COX-2的表达强度均有明显差异,米非司酮高剂量组低于低剂量组(P<0.01)和对照组(P<0.01),低剂量组在位内膜COX-2的表达强度也明显低于正常对照组(P<0.01)。2)米非司酮高剂量组异位内膜COX-2的表达强度低于正常对照组(P<0.01)和低剂量组(P<0.01),而低剂量组异位内膜COX-2的表达强度和对照组比较差异无显著性。3)米非司酮高剂量、低剂量组异位内膜COX-2的表达强度均明显高于在位内膜(P<0.01),而对照组在位、异位内膜COX-2的表达强度差别无显著性。结论:米非司酮治疗EMS可能与降低COX-2的表达有关。

Aim： To explore the effect of mifepristone on the expression of COX - 2 in rat eutopic and ectopic endometrium. Methods：Endometriotic rats were randomly divided into control group and mifepristone group （including low dose and high dose groups）. 4 weeks after gastrogavage with tuwen -80 only （control group） or tuwen -80 plus mifepristone 0. 62 mg/d （low dose group） or 1.24 mg/d （high dose group）, all rats were killed and tissues of ectopic and eutopic endometrium were removed for detecting COX - 2 expression via immunohistochemistry （S - P method） . Results： I ） In the eutopic endometrium, the expression intensity of COX -2 in the high dose group was lower than that in the low dose group and the control group （P 〈0. 01 ）. The expression intensity of COX -2 in the low dose group was lower than that in the control group （ P 〈 0. 01 ）. 2） In the ectopic endometrium, the expression intensity of COX- 2 in the high dose group was lower than that in the low dose group （P 〈0. 01 ）and the control group （P 〈0. 01 ）, but it was no markedly difference between the low dose group and the control group. 3）In both high dose and low dose groups, the expression intensity of COX - 2 in the ectopic endometrium was higher than that in the eutopic endometrium （P 〈0. 01 ）, but in the control group, it was no significantly difference in the expression intensity of COX -2 between the ectopic endometrium and the eutopic endometrium. Conclusion：The treament effect of mifepristone on EMS might be related with decreased expression of COX -2.