外源性L-精氨酸对小鼠日本血吸虫病肝脏内I型和Ⅲ型胶原的影响

Effects of L-arginine on collagen I and collagen Ⅲ in the hepatic tissues of mice with Schistosoma japonicum

目的:动态观察外源性L-精氨酸对小鼠日本血吸虫病肝脏内I型和Ⅲ型胶原的影响,探讨其治疗血吸虫病肝纤维化的可能性。方法:随机将54只健康小鼠分为正常组、感染组和L-精氨酸组。感染组和L-精氨酸组小鼠经腹部皮肤感染日本血吸虫尾蚴。L-精氨酸组于感染后第6周将1 g/L的L-精氨酸水溶液给小鼠饮用。于感染后的第9、11、13、15周每组随机挑选6只处死取肝脏做病理观察;并对随机选取的30个视野下I型和Ⅲ型胶原面积及两者之比值作定量分析。结果:感染组与L-精氨酸组小鼠肝脏内I型和Ⅲ型胶原面积在感染第9周后逐渐减小,I型和Ⅲ型胶原面积比值变化较大;L-精氨酸组I型和Ⅲ型胶原面积比感染组减小明显(P<0.05)。结论:L-精氨酸具有减少日本血吸虫病肝脏组织内I型胶原和Ⅲ型胶原的作用。

Aim： Through observing the effects of L- Arginine on Collagen Ⅰ and Collagen Ⅲ in the hepatic tissues of mice with Schistosoma japonicum, to explore its action of curing liver fibrosis. Methods： 54 mice were randomly divided into normal group （6 mice）, infective group （24 mice） and L- Arginine treatment group （24 mice）. Each mouse both in infective group and L- Arginine treatment group was infected with cercaria of Schistosoma japonicum through its skin of abdomen. 1 g/L L - Arginine solution was freely drunk by the mice of L - Arginie treatment group in the 6^th week after infected. 6 mice from each group were killed at 9^th, 11^th, 13^th and 15^th weeks respectively after infected and their livers were collected to undergo staining and pathologic observation by micropolarimete. The areas of Collagen Ⅰ and Collagen Ⅲ in hepatic fibroses were measured using computer image analysis software. Results： the areas of Collagen Ⅰ and Collagen Ⅲ in the hepatic tissues of mice both infective group and L - Arginine treatment group reduce generally, and the change of the ratio of Collagen Ⅰ and Collagen Ⅲ is large, and the areas of Collagen Ⅰ and Collagen Ⅲ in L- Arginine treatment group were reduced more obviously than in infective group （ P 〈 0. 05）. Conclusion： L - Arginine has effects on promoting decrease of Collagen Ⅰ and Collagen Ⅲ in the hepatic tissues of mice with Schistosoma japonicum.