放射性核素^(99m)Tc-奥曲肽体内生物分布的研究

The study of biodistribution of ^(99m)Tc radiolabeled octreotide in rats and human

目的:探讨99mTc-奥曲肽在生物体内与生长抑素受体结合的影像分布特性,为生长抑素受体显像提供实验依据。方法:利用薄层层析法计算99mTc-奥曲肽标记率与放化纯度。3只SD大鼠经静脉注射99mTc-奥曲肽后,在不同时间内显像并测定心血池放射性计数,计算奥曲肽血药浓度;3名志愿者静脉注射99mTc-奥曲肽后在不同时间内显像并观察人体内生长抑素受体分布。结果:99mTc-奥曲肽的放化纯度可以满足显像试验要求,静脉注射后在血中迅速进入器官组织,在肝脏中有较高浓度的聚集,主要经尿路排泄。结论:99mTc-奥曲肽在血液内清除速度快,主要在肝脏浓聚,并通过泌尿系统排泄,显像结果证明其具有很好的显像特性。

Aim： To evaluate the characteristic of receptor - binding of somatostain receptor imaging agent ^99mTc radiolabeled octreotide in vitro and provides the information for somatostain receptor imaging experiment. Methods： Label rate and purity were calculated with thin - layer chromatography. Radioactivity biodistribution assay was performed in 3 SD rats and clinical somatostatin receptor imaging was performed in three healthy volunteers after intravenous injection ^99mTc radiolabeled octreotide. Results： The radiochemical purity of ^99mTc radiolabeled octreotide was satisfied under the imaging requirement. ^99mTc radiolabeled octreotide removed from blood into organ and tissue and was high concentrated in the liver. The tracer excretes mostly from the urinary tract after injection. Conclusion： The blood radioactivity of ^99mTc radiolabeled octreotide decreased rapidly, the clear image indicates that the ^99mTc radiolabeled octreotide is a kind of nice imaging agent.