栗疫菌(Endothia parasitica)致病毒素的初步研究

The Preliminary Research on Phytotoxin in Endothia parasitica Metabolic Product

栗疫菌(Endothia parasitica)引起的板栗疫病是森林植物检疫对象之一。病原菌在侵染植物的过程中产生的毒素一向被认为是致病的重要因子。本实验以PD液体培养基为基础,通过震荡和静止两种培养方式,采用浸渍法和针刺法对板栗叶片和小枝进行了生物测定。研究显示板栗小枝和叶片可产生典型的症状,证明栗疫菌能产生具生物活性的毒性物质(毒素),PD培养液适于菌株的生长和毒素的产生。震荡培养比静止条件更利于产毒,浸渍法处理后第二天板栗小枝和叶片都表现典型的病斑和萎蔫症状,针刺法处理后第一天便表现出典型症状,说明针刺法比浸渍法效果更明显、叶片比小枝更敏感。

Chestnut blight caused by Endothia parasitica, is one of forest plant quarantine pests. Toxin is one of important pathogenic factors to plants. Shake culture and still method were applied in this experiment and the substrate was PD. Two bioassay methods were conducted in this experiment, namely dipping method and needle puncture. The experiment show that twigs and leaves indicate distinct symptom. So Endothia parasitica can produce bioactive phytotoxin, PD medium filtrate is suitable for isolate growth and toxin production, shake culture is better than still method; the second day with dipping method twigs and leaves indicates pathological changes and wilt, the first day with needle puncture plant indicates symptom. So needle puncture is better than dipping method and leaves are more sensitive than twigs.