运动训练对急性脑梗死大鼠行为学及bFGF、TGF的影响

The rehabilitative effects and the expression of bFGF and TGF in rats after acute cerebral infarction

目的:观察运动训练对急性脑梗死大鼠脑功能恢复及碱性成纤维细胞生长因子(bFGF)、转化生长因子(TGF)的影响。方法:采用抽签法将30只大鼠随机分为3组,A组(假手术组)、B组(造模组)、C组(运动训练组),采用血管内栓线法制备脑缺血动物模型,术后24h、3d、7d分别进行Bederson神经功能评分、平衡木、转棒、网屏测评及bFGF、TGF的测定。结果:与A组比较,B、C组的Bederson评分各时间点均有极显著性差异(P<0.01),随着时间的延长,A组的各项指标很快恢复,B、C组功能改善远不及A组,差异有显著意义(P<0.01);C组平衡木评分在术后3d即与B组差异具有显著性(P<0.05);术后7d除Bederson评分外,C组与B组比较差异具有显著性意义(P<0.01或P<0.05);A组与B组术后7d皮质及海马部位均有少量的bFGF及TGF表达,两组间比较无显著性差异(P>0.05),C组的bFGF及TGF表达明显升高(P<0.01)。结论:运动训练能提高脑梗死大鼠平衡、行走及抓握能力,增加患肢的肌力,诱导缺血周边区bFGF及TGF的表达。

Objective： To observe the effects of rehabilitation training on behavior and expression of bFGF,TGF in rats after acute cerebral infarction. Method： Thirty male Wistar rats were randomly divided into 3 groups,group A （sham operation）, group B（model）and group C（rehabilitation）. Animal model were made through middle cerebral artery obstruction（MCAO）, Bederson neural function was scored and balancing wood,rotating bar,and net screen were tested at 24 hours, 3rd and 7th day after operation. The expression of bFGF and TGF of each group were also tested at 7th day after operation.Result：Compared with group A,Bederson neural function scores of group B and C indicated significant differences at each time （P〈0.01）. The handicapped ability of grasping,walking and coordinating of all groups happened at 24 hours after operation. However as time went on, every function of group A restored and that of group B or C improved partly,but showed significant differences compared with group A（P〈0.01）, Three days after operation,balancing wood test of group C was better than that of group B（P〈0.05）. Seven days after operation, besides Bederson neural function score,there were significant differences between group C and B （P〈0.01 or P〈 0.05）. Seven days after operation,there were a few bFGF and TGF expressions in hippocampus and cortex areas of group A and B,and there was no difference between this two groups （P〉0.05）,while those of group C greatly in- creased,compared with group A and B （P〈0.01）.Concluslon：The rehabilitation therapy can improve the MCAO rats＇ ability of balancing, grasping, walking and strengthen the myodynamics,as well as induce the expression of bFGF and TGF in cortex and hippocampus.