节瘤拟杆菌PCR检测方法的初步应用

Initial Application of a PCR for Detection of D.nodosus

本研究在建立节瘤拟杆菌PCR检测方法的基础上,将PCR方法用于临床样品的检测。PCR检测发现制约PCR直接检测临床样品检出率的主要因素是临床样品中细菌外的抑制物;采自趾叉皮炎的临床样品16份PCR检测呈阳性,趾叉皮炎是腐蹄病的初期感染症状;而其它蹄病后期症状临床样品PCR检测均为阴性,表明PC R检测方法适宜腐蹄病的早期诊断。

The developed PCR technique were evaluated with directly lysate of samples from lesions in footrot,the results found PCR test was accurate and successful corresponding with DNA sequencing and microscope observation to sample from interdigital dermatitis in footrot; which is early syndrome of footrot in ruminant, so the PCR test was practised to detect those sample from early lesion.From the results ,it will become a practical method to diagnosis of footrot in an early infection.