烤烟叶片发育过程中类胡萝卜素组分含量变化研究

Research on Metabolism of Flue-Cured Tobacco Leaf Carotenoids During Its Growth Development

利用反相高效液相色谱法和分光光度计法,对不同部位烤烟叶片发育过程中类胡萝卜素组分含量进行了测定.结果表明,烤烟发育过程中类胡萝卜素含量随生育时期的推进而逐渐下降,但不同部位叶片的类胡萝卜素组分含量有明显差异.中部叶片的β胡萝卜素、新黄质、紫黄质、叶黄质、叶黄素含量变化比较平稳.与下、中部叶片相比,上部叶片发育后期其组分含量均较高,其中β-胡萝卜素含量最高,为16.1 mg.g-1.

The contents of carotenoids in different parts of flue-cured tobacco leaf during its growth development were determined by RP-HPLC. The results show that the contents of cartenoids decreased gradually during the flue-cured tobacco leaf growth development, and leaves of different parts of tobacco had different contents of carotenoids, β-carotene, neoxanthin, violaxanthin, lutein and xanthophylls contents remained stable in the middle part of the flue-cured tobacco leaves during the development. Compared with lower part tobacco leaves and middle part tobacco leaves, upper part flue-cured tobacco leaves during its latter stage have higher contents of β-carotene, neoxanthin, violaxanthin, lutein and xanthophylls, and 16.1mg·g^-1 β-carotene as the highest.