缓激肽促进大鼠肾系膜细胞增殖和纤维连接蛋白表达被引量：1

Bradykinin stimulates proliferation and fibronectin expression in cultured rat renal mesangial cells

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摘要目的:观察缓激肽(BK)对大鼠肾系膜细胞增殖和纤维连接蛋白表达的影响。方法:采用体外培养的大鼠肾系膜细胞,应用四甲基偶氮唑盐(MTT)比色法检测细胞增殖情况;应用Westernblotting观察肾系膜细胞纤维连接蛋白的表达。结果:缓激肽促进肾系膜细胞增殖,且具有剂量依赖性。缓激肽和转化生长因子-β1皆显著增加系膜细胞纤维连接蛋白表达,并具有协同效应。结论:缓激肽可能直接促进体外培养的系膜细胞增殖和纤维连接蛋白表达,从而加重对肾小球的损伤。 Objective： To investigate the direct effect of hradykinin（BK） on proliferation and fibronectin expression in cultured rat mesangial cells and whether the effects of BK and transforming growth factor-β1 （TGF-β1） on fibronectin levels are synergistic. Methods： Mesangial cell proliferation was assessed by MTT assay, and fibronectin expression in mesangial cells was investigated by Western blotting. Results： In quiescent mesangial cell cultures （0.5% serum for 24 hr）, BK stimulated cell proliferation in a dose dependent fashion. Both BK and TGF-β1 induced a significant increase in mesangial cell fibronectin levels. Furthermore, the effects of BK and TGF-β1 on fibronectin levels were synergistic. Conclusion： These results suggest that BK directly stimulates proliferation and fibronectin expression in cultured mesangial cells, thus promoting glomerular injury.

作者刘霞

机构地区南通大学医学院病理生理学教研室

出处《交通医学》 2006年第6期653-654,657,共3页 Medical Journal of Communications

基金南通市社会发展项目(S0020)

关键词肾系膜细胞缓激肽细胞增殖纤维连接蛋白 Renal mesangial cells Bradykinin Cell proliferation Fibronectin

分类号 Q491 [生物学—生理学]

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1Johnson RJ,Alpers CE,Yoshimura A,et al.Renal injury from angiotensin Ⅱ-mediated hypertension[J].Hypertension,1992,19(5):464.
2Schnaper HW,Hayashida T,Hubchak SC,et al.TGF-beta signal transduction and mesangial cell fibrogenesis[J].Am J Physiol Renal Physiol,2003,284(2):F243.
3Schanstra JP,Neau E,Drogoz P,et al.In vivo bradykinin B2 receptor activation reduces renal fibrosis[J].J Clin Invest,2002,110(3):371.
4Tan Y,Wang B,Keum JS,et al.Mechanisms through which bradykinin promotes glomerular injury in diabetes[J].Am J Physiol Renal Physiol,2005,288(3):F483.
5El-Dahr SS,Dipp S,Baricos WH.Bradykinin stimulates the ERK--》Elk-1--》Fos/AP-1 pathway in mesangial cells[J].Am J Physiol,1998,275(3 Pt 2):F343.
6Alric C,Pecher C,Schanstra JP,et al.Bradykinin-induced inhibition of cell proliferation and tyrosine kinase activity in rat mesangial cells[J].Int J Mol Med,2000,5(1):85.
7EL-Dahr SS,Dipp S,Yosipiv Ⅳ,et al.Bradykinin stimulates c-fos expression,AP-l-DNA binding activity and proliferation of rat glomerular mesangial cells[J].Kidney Int,1996,50(6):1850.