摘要
以聚丙烯酰胺凝胶电泳检测武汉地区247份腹泻患儿粪便样本,筛选出A组轮状病毒阳性样本67份,阳性率为27.12%。通过一步多重RT-PCR技术对筛选出的阳性样本进行VP7基因分型研究,其中G 1型10例(14.93%),G 3型46例(68.66%),G 1与G 3混合感染型5例(7.46%),6例(8.95%)未能分出型别。分析表明,武汉地区A组轮状病毒以G 3型为主要流行基因型,同2002-2003年相比,G 3型比例下降,G 1型比例上升。
From March to November in 2006 in Wuhan, China, we collected 247 fecal specimens of pediatric patients suffering from diarrhea. With PAGE （Polyacarylamide Gel Electrophoresis）, we identified 67 specimens, 27.12% of the total specimens, as positive group A rotavirus. Through onestep multiplex RT-PCR technology （RT： reverse transcription; PCR： polymerase chain reaction）, we tested the VP7 （viral protein 7） genotypes of the positive samples. Among them 46 （68.66%） are recognized as G3,10 （14.93%） are type G1,5 （7.46%） are admixtures of G1 and G3, and the rest 6 （8. 95%） are unknown. Epidemic statistics shows that during the investigation period in 2006, G3 was the prevalent type in Wuhan. Compared with the period from 2002 to 2003, G3 decreased and G1 increased in 2006.
出处
《黄冈师范学院学报》
2006年第6期69-72,共4页
Journal of Huanggang Normal University
