HBV感染对原发性肝细胞癌生物学行为及Survivin表达的影响

Influenec of HBV infection on biological behaviours of hepatocellular carcinoma(HCC) and expression of survivin protein

目的:探讨在原发性肝细胞癌(hepatocellular cancinoma,HCC)中乙型肝炎病毒(hepatitis Bvirus,HBV)感染对肿瘤生物学行为及Survivin蛋白表达的影响。方法:利用血清学指标检测61例HCC的HBV感染情况,采用辣根酶标记生物素链霉卵白素(SP)检测癌组织中survivin蛋白的表达。结果:39例HBV感染HCC中Survivin蛋白表达率为69.23%(27/39),22例非HBV感染表达率为31.81%(8/22),有显著性差异(P<0.05)。在HCC中,HBV感染与肿瘤的Edmondson分级、AFP增高、门静脉癌栓形成、肝内转移及转氨酶升高相关(P<0.05)。结论:HBV感染可能与Survivin共同作用导致HCC的发生及发展。

Objective： To study the influences of HBV infection on both biological behaviors of hepatocellular cancinoma （HCC） and expression of survivin protein. Methods： The infectious conditions of HBV were determined by serum tests for 61 cases of patients with hepatocellular cancinoma. Meanwhile, the expression of survivin protein in hepatocellular carcinoma tissues was detected by streptavidin-perosidase （S-P） method. Results： The expression rates was 69.23% （27/3） and 31.81% （7/22） in 39 cases with hepatocellular cancinoma infected by HBV and 22 cases without infected by HBV （P〈0.05）. Among the patients with HCC, the states of HBV infection was high positively correlated with Edmondson grades, the elevation of serum α-fectoprotien （AFP）, the formation of intraportal carcinoma embolism, intrahepatic metastasis, and elevation of serum γ-glutamyl transferase （P〈0.05）. Conclusion： The both HBV infection and survivin protein may have cooperative effects for the occurrence and development of HCC.