期刊文献+

肥大细胞在慢性肝病发生中的作用 被引量:5

Role of mast cells in development of chronic liver diseases
下载PDF
导出
摘要 目的:探讨肥大细胞(m ast cell,MC)在慢性肝病中的作用及乙型肝炎病毒(HBV)感染是否引起慢性肝病中MC数量增加。方法:本研究包括正常组(NL)8例、慢性肝炎组(CH)30例、肝硬化组(LC)43例、肝癌组(HCC)49例。采用甲苯胺蓝染色和免疫组织化学染色观察130例人肝组织中肥大细胞的密度和分布特征。另外,采用免疫组织化学染色定性检测各组HBsAg,HBcAg的表达。结果:各肝病组中(肝炎组、肝硬化组、肝癌组)肥大细胞密度比正常组显著增加(P<0.05);肝硬化组、肝癌组中MC密度均比慢性肝炎组显著增加(P<0.05);但肝硬化组与肝癌组之间差异无统计学意义(P>0.05)。MC分布以结缔组织区域多见。本组病例中肥大细胞密度与HBV感染无关。结论:肥大细胞可能参与慢性肝病发生发展过程并发挥重要作用,但其数量增加可能与HBV感染无直接关系。 Objective To investigate the role of mast cells in chronic liver diseases and the relation between the number of mast cells and hepatitis B virus (HBV) infection. Methods The density and characteristic of distribution of the mast cells in 130 human liver tissue specimens, including normal livers ( NL, n = 8), chronic hepatitis ( CH, n = 30), liver cirrhosis ( LC, n = 43 ) and hepatocellular carcinoma (HCC, n = 49) were observed by using toluidine blue staining and immunohistochemistry. Additionally, HBsAg and HBcAg in all cases were detected qualitatively by using immunohistochemistry. Results The densities of mast cells in diseased liver tissue were significantly higher than those in normal liver tissue (P 〈 0.05 ), and the densities of mast cells in LC and HCC were significantly higher than those in CH (P 〈 0.05 ). But no significant difference was found between the mast cells densities in LC and HCC (P 〉 0.05 ). The distribution of mast cells was rich in the region of connective tissue. Additionally, the densities of mast cell in liver tissues did not associate with HBV infection. Conclusion Mast cells may participate in the progression of chronic liver diseases and play an important role, but the increases in number of mast cells may not directly associate with HBV infection.
出处 《国际病理科学与临床杂志》 CAS 2006年第6期461-465,F0002,共6页 Journal of International Pathology and Clinical Medicine
基金 国家自然科学基金(30270601)
关键词 肥大细胞 肝脏 乙型肝炎病毒 mast cells liver hepatitis B virus
  • 相关文献

参考文献34

  • 1Fodinger M,Fritsch G,Winkler K,et al.Origin of human mast cells:Development from transplanted hematopoietic stem cells after allogenic bone marrow transplantation[J] Blood,1994,84(9):2954-2959.
  • 2Galli S J.Mast cells and basophils[J].Curr Opin Hematol,2000,7(1):32-39.
  • 3Gaca M D,Pickering J A,Arthur M J,et al.Human and rat hepatic stellate cells produce stem cell factor:a possible mechanism for mast cell recruitment in liver fibrosis[J].J Hepatol,1999,30(5):850-858.
  • 4Farrell D J,Hines J E,Walls A F,et al.Intrahepatic mast cells in chronic liver diseases[J].Hepatology,1995,22(4 Pt 1):1175-1181.
  • 5Nakamura A,Yamazaki K,Suzuki K,et al.Increased portal tract infiltration of mast cells and eosinophils in primary biliary cirrhosis[J].Am J Gastroenterol,1997,92(12):2245-2249.
  • 6Terada T,Matsunaga Y.Increased mast cells in hepatocellular carcinoma and intrahepatic cholangiocarcinoma[J].J Hepatol,2000,33(6):961-966.
  • 7Jeong W I,Jeong D H,Do S H.Mild hepatic fibrosis in cholesterol and sodium cholate diet-fed rats[J].J Vet Med Sci,2005,67(3):235-242.
  • 8Matsunaga Y,Terada T.Mast cell subpopulations in chronic inflammatory hepatobiliary diseases[J].Liver,2000,20(2):152-156.
  • 9贾晋斌,韩德五,崔慧林,杨美林,闫晋平,马学惠.肥大细胞在肝纤维化发生发展过程中的作用及其与TNFα的关系[J].中华肝脏病杂志,1998,6(4):235-237. 被引量:8
  • 10Sugihara A,Tsujimura T,Fujita Y,et al.Evaluation of role of mast cells in the development of liver fibrosis using mast cell-deficient rats and mice[J].J Hepatol,1999,30(5):859-867.

二级参考文献5

  • 1Alexandrakin MG, Kyriakou DS, Seretakis D, et al. Inhibitory effect of retinoic acid on proliferation, maturation and tryptase level in human leukemic mast cell (HMC-1)[J]. Int J Immunopathol Pharmacol, 2003, 16: 43-47.
  • 2Esposito T, Kleeff J, Bischoff SC, et al. The stem cell factor-c-kit system and mast cells in human pancreatic cancer [J].Lab Invest, 2003, 82: 1481-1492.
  • 3Horny HP, Greschniod A, Jordan JH, et al. Chymase expression bone marrow mast cells in mastocytosis and myeldys plastic syndromes: an immunohistochemical and morphometric study [J]. J Clin Pathol, 2003, 56: 103-106.
  • 4Norrby K. Must cells and angiogenesis. ADMIS, 2002, 110:355-357.
  • 5韩德五,马学慧,赵元昌.肝硬化动物模型的研究[J]山西医药杂志,1979(04).

共引文献8

同被引文献56

引证文献5

二级引证文献17

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部