人胎盘滋养层细胞培养与体外hCG释放的研究

STUDY ON TROPHOBLASTIC CELLS FROM HUMAN TERM PLACENTAE AND RELEASE OF HCG IN VITRO

本研究的目的是了解细胞滋养层细胞和合胞体滋养层细胞体外分化和生物学特性。方法:采用酶消化和Percoll密度梯度离心法,对人足月胎盘细胞滋养层细胞进行分离、纯化和体外培养。采用放射免疫法(RIA)检测细胞培养上清液hCG含量的变化。结果:经分离和纯化的细胞滋养层细胞在体外培养中生长良好,通过细胞分裂和融合形成合胞体滋养层细胞,随着合胞体滋养层细胞的生长,细胞培养上清液中hCG含量显著升高。我们认为从胎盘中分离和纯化的细胞滋养层细胞在体外培养中可分化和融合形成合胞体滋养层细胞,体外hCG含量的增加与合胞体滋养层细胞生长有关。

The objective of this study was to determine the differentiation and biological characteri-zation of cytotrophoblastic celis and syncytiotrophoblastic celis in vitro. Methods: We purified cytotrophoblastic cells isolated from human term placentae by enzymic digestion and percoll gra-dient centrifugation, hCG from culture media has been detected by RIA. ResultS: Cytotrophob-lastic celis isolated and purified from human placentae growth in vitro and transformated into the functional syncytiotrophoblastic celis through a process of differentiation and cell fasion. hCG RIA of the culture media revealed asignificant increase in secretion. paralleling the gro-wth of syncytial cytotrophoblasts. We conclude that cytotrophoblastic celis isolated from human term placentae may differentiate and fuse to form functional syncytiotrophoblastic cells in vitro. The increase of hCG secretion is related to the growth of syncytiotrophoblastic cells.