萝卜游离小孢子培养研究初报

Studies on culturing the isolated microspores of radish(Raphanus sativus L.)

以30份夏、秋萝卜品种为试材,对游离小孢子培养技术进行研究,结果秋萝卜有4份获得了胚状体,研究表明:基因型对小孢子培养起着决定性作用;低温预处理花蕾可显著提高出胚量;单核晚期的小孢子最适宜小孢子培养;33℃高温热激培养48 h产胚量最高;最适合的诱导培养基是1/2 NLN-12,附加0.05mg.L-16-BA+0.2 mg.L-1NAA+0.02 mg.L-1KT+5 mg.L-12,4-D+5 mg.L-1AgNO3+0.05mg.L-1AC。

The culturing technique of isolated microspore was studied by using of thirty Cultivars of summer and autumn type radish as donor plants. The embryoids had been obtained from four cultivars of autumn type radish. The results showed the genorype of donor plants played a decisive role in the culture of isolated microspore, somatic embryo yields were significantly increased by low temperature prerreatment of flower buds. Furthermore, the optimum stage of microspore for the cultivation was uninuclease anaphase, maximum somatic embryo yields was obtained with the heat-shock treatment for 48h at 33℃. The 1/2 NLN- 12 medium, containing 0.05 mg·L^-1 6- BA, 0. 2 mg·L^-1 NAA, 0.02mg·L^-1 KT, 5mg·L^-1 2, 4-D, and 5mg·L^-1 AgNO3, 0.05 mg· L^-1 AC, wasthe optimal medium for somatic embryo inducement.