酶解花生蛋白影响Cu^(2+)吸收利用的体外实验研究

以花生蛋白经体外模拟消化所得的消化物为研究对象,研究酶解花生蛋白对铜生物效价的影响。结果表明,酶解蛋白与铜的结合率低于天然蛋白与铜的结合率,随着Cu2+的增加,铜的结合率也有所提高;Ca2+和Fe2+的加入能降低模拟消化物与Cu2+的结合率。实验表明,花生蛋白经模拟胃液和胰液消化,可影响Cu2+的生物效价,其与Cu2+的结合能力和Cu2+浓度、Ca2+、Fe2+的存在有关。

Peanut protein was studied in an in vitro digestion system composed of pepsin and trypsin on its effect on the bioavailability of Cu^2＋. The binding rate of copper in enzymic hydrolyzed peanut protein was lower than in native peanut protein; with the increasing copper content the binding rate was increasing. Ca^2＋ and Fe^2＋ added can reduce the binding capability between hydrolyzed protein and Cu^2＋ Results showed binding capability between Cu^2＋ and hydrolyzed peanut protein by pepsin and trypsin in vitro was correlated to Cu^2＋ concentration, added Ca^2＋ and Fe^2＋. The biological value of copper was influenced by enzymic peanut protein hydrolyzate.