摘要
采用反相液相色谱法同时测定降糖样品中的盐酸二甲双胍、盐酸苯乙双胍、格列吡嗪、格列齐特、格列本脲、格列美脲等六种降血糖西药成分。色谱柱为AgilentC18柱,流动相为乙腈-0.02%磷酸溶液,梯度洗脱,波长229nm,流速1.0mL/min。17分钟可实现六组分的完全分离,各组分在一定范围内有良好的线性关系。具有快速简单、分离效果好、回收率高等优点。
An RP-HPLC method was proposed to the simultaneous determination of six components： metformin hydrochloride, phenformin hydrochloride,glipizide, gliclazide, glibenclamide and glimepiride, which were western medicines of reducing blood sugar. The six components could be separated completely in 17 minutes on a Agilent C18 column and then detected at 229 nm, using acetonitrile and 0.02% phosphoric acid with gradient elution as the mobile phase at flow rate 1.0 ml/min.Linear calibration graphs for each component were obtained within their range. This method proved to be simple, rapid with good seperation and good recoveries.
出处
《现代科学仪器》
2006年第6期89-91,共3页
Modern Scientific Instruments
