免疫胶体金试剂的制备及应用胶体金免疫电镜技术检测戊型肝炎病毒

Preparation of Immunocolloidal Gold and Its Application to the Detection of Hepatitis E Virus

报道了用胶体金免疫电镜技术检测戊型肝炎病毒（ＨＥＶ）的方法。实验采用１０ｎｍ胶体金标记纯化兔抗鼠ＩｇＧ制备了免疫胶体金探针试剂。用本法从戊型肝炎患者粪便提取物中检出ＨＥＶ呈球形，直径３２±５ｎｍ。此方法具有快速、灵敏、直观等特点，在ＨＥＶ生物学性质研究中，有一定的应用价值。

this paper reports that the method of colloidal gold immunoelectronmicroscopy was used to detect hepatitis E virus (HEV).In this system,10nm colloidal gold labeled rabbit anti mouse IgG (RAMIgG) which was used as immunoprobe.HEV particles were detected from stools of HE patients is spherical,the diameter is 32±5 nm.The method has the distinguishing features of simplicity,rapidity,sensitivity and directly perceived through the senses.It is practical and applicable in research of HEV biological character.